Embryonic and postnatal development of the rat renal interstitium
Embryonic and postnatal development of the rat renal interstitium
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DOI：
10.1007/s004290050070
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发表时间：
1997-06-01
期刊：
影响因子：
--
通讯作者：
Alcorn, D
中科院分区：
文献类型：
--
作者：
Maric, C;Ryan, GB;Alcorn, D
虽然抗高血压，结构和功能的作用已经提出了成人肾脏中的肾髓质细胞，很少有人知道它在肾脏发育中的作用。通过光学显微镜、透射和扫描电子显微镜以及免疫细胞化学，在整个发育过程中，在出生前胎龄E14-E21和出生后0-28天对大鼠肾脏进行了研究。肾髓质间质细胞早在胚胎E14天就已出现，在输尿管芽的分支周围形成疏松、有序的网状结构。在第一肾单位结构发育的同时，肾髓质间质细胞在集合管周围呈同心圆状排列。随着肾小管和血管从皮质生长到髓质，这种排列导致肾小管、血管和集合管之间的间质细胞出现特征性的“阶梯梯级"。肾髓质间质细胞在发育早期即与肾小管、血管和集合管的基底膜紧密粘附。在重塑过程中，肾髓质间质细胞与管状结构之间缺乏接触，如Henle袢的发夹弯曲。在这些病灶层粘连蛋白，基底膜糖蛋白特异性定位于细胞内上皮网站，而在更发达的地区，层粘连蛋白仅限于上皮基底膜。与更成熟的结构相关，层粘连蛋白也定位于肾髓质间质细胞的细胞内颗粒。因此，肾髓质间质细胞存在于肾小管在髓质中的重新定位之前，并且似乎与肾小管在髓质中的重新定位积极相关，从而将自身确立为肾脏发育过程中不可或缺的一部分。
Whilst antihypertensive, structural and functional roles have been proposed for the cells of the renomedullary interstitium in the adult kidney, little is known about its role in renal development. Rat kidneys were studied throughout development, prenatally at gestational ages E14-E21 and postnatally at 0-28 days, by light microscopy, transmission and scanning electron microscopy and following immunocytochemistry. Renomedullary interstitial cells were observed as early as embryonic day E14, forming a loose, orderly network around branches of the ureteric bud. Paralleling the development of the first nephron structures, renomedullary interstitial cells were arranged in a concentric circular manner around collecting ducts. Following tubular and vascular growth from the cortex into the medulla, this arrangement resulted in the characteristic `rungs of a ladder' appearance of interstitial cells between tubules, blood vessels and the collecting ducts. Renomedullary interstitial cells were closely adherent to basement membranes of tubules, blood vessels and collecting ducts from early in development. Contacts were absent between renomedullary interstitial cells and tubular structures in the process of remodelling, such as the hair-pin bends of the loops of Henle. At these foci laminin, a basement membrane glycoprotein was specificially localised to intracellular epithelial sites, whereas in more developed areas, laminin was restricted to epithelial basement membranes. Associated with the more mature structures, laminin was also localised to intracellular granules of renomedullary interstitial cells. Thus, renomedullary interstitial cells are present prior to and appear to be actively associated with tubule repositioning in the medulla, establishing themselves as integral to the process of renal development.