A role for fission yeast Rab GTPase Ypt7p in sporulation
A role for fission yeast Rab GTPase Ypt7p in sporulation
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DOI：
10.1247/csf.30.43
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发表时间：
2005-12-01
影响因子：
1.5
通讯作者：
Shimoda, C
中科院分区：
文献类型：
--
作者：
Kashiwazaki, J;Nakamura, T;Shimoda, C
Ypt7p是Rab7 GTdR的裂殖酵母（裂殖酵母）同源物，介导内体与液泡的融合和同型液泡融合。在这里，我们报告Ypt7p在孢子形成中起着重要作用。大多数ypt7三角洲子囊产生少于四个孢子，这显然是不成熟的，发芽频率低。此外，ypt7 δ细胞在前孢子膜的发育中有缺陷。孢子形成细胞中的小泡发生广泛的同型液泡融合，形成几个大的隔室，占据整个子囊细胞质。这种广泛的液泡融合依赖于Ypt7p。
Ypt7p, a fission yeast (Schizosaccharomyces pombe) homologue of Rab7 GTPase, mediates fusion of endosomes to vacuoles and homotypic vacuole fusion. Here, we report that Ypt7p plays important roles in sporulation. Most ypt7 Delta asci produced less than four spores, which were apparently immature and germinated at low frequency. Furthermore, ypt7 Delta cells were defective in development of the forespore membranes. Vacuoles in sporulating cells were found to undergo extensive homotypic vacuole fusion to form a few large compartments occupying the entire cytoplasm of asci. This extensive vacuole fusion depended on Ypt7p.