Superoxide dismutase induces differentiation of friend erythroleukemia cells
Superoxide dismutase induces differentiation of friend erythroleukemia cells
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DOI：
10.1002/jcp.1041390220
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发表时间：
1989-05
影响因子：
5.6
通讯作者：
B. Beckman;A. Balin;R. G. Allen
中科院分区：
文献类型：
--
作者：
B. Beckman;A. Balin;R. G. Allen
Friend红白血病细胞（FELC）作为细胞分化的模型系统，因为这些细胞可以被各种化学试剂触发分化。用经典的分化诱导剂β-双乙酰胺（HMBA）处理，刺激超氧化物歧化酶（SOD）活性，其与HMBA诱导的分化平行增加。此外，FELC被证明是分化的反应，加入脂质体含有SOD。用含有D-氨基酸氧化酶或黄嘌呤氧化酶、过氧化异丙苯或超氧化钾的脂质体进行氧化处理也诱导分化，而抗氧化剂如α-生育酚、丁基化羟基甲苯或β-胡萝卜素不诱导分化。此外，HMBA诱导的分化被抑制与抗氧化剂的治疗。
Friend erythroleukemia cells (FELC) served as a model system for cell differentiation because these cells can be triggered to differentiate by a variety of chemical agents. Treatment with the classical inducer of differentiation, hexamethylene bisacetamide (HMBA), stimulated superoxide dismutase (SOD) activity, which increased in parallel with HMBA‐induced differentiation. Furthermore, FELC were shown to differentiate in response to the addition of liposomes containing SOD. Oxidative treatment with Iiposomes containing D‐amino acid oxidase or xanthine oxidase, cumene peroxide, or potassium superoxide also induced differentiation, whereas antioxidants such as a‐tocopherol, butylated hydroxytoluene, or p‐carotene did not induce differentiation. Also, HMBA induction of differentiation was suppressed by treatment with antioxidants.