A non-structural protein encoded byRice Dwarf Virustargets to the nucleus and chloroplast and inhibits local RNA silencing
A non-structural protein encoded byRice Dwarf Virustargets to the nucleus and chloroplast and inhibits local RNA silencing
复制标题
水稻矮缩病毒编码的一种非结构蛋白靶向细胞核和叶绿体并抑制局部 RNA 沉默。
DOI：
10.1007/s11427-019-1648-3
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发表时间：
2020-04-15
影响因子：
9.1
通讯作者：
Li, Yi
中科院分区：
文献类型：
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作者：
Gao, Feng;Zhao, Shanshan;Li, Yi
RNA沉默是植物和动物中一种有效的抗病毒机制。作为一种反防御措施，迄今为止研究的许多病毒都编码一种或多种 RNA 沉默 (VSR) 病毒抑制因子。在后一种情况下，病毒编码的不同 VSR 如何发挥沉默作用仍有待充分了解。我们之前表明，植物呼肠病毒、水稻矮缩病毒（RDV）的非结构蛋白 Pns10 具有 VSR 功能。在这里，我们提供了另一种非结构蛋白 Pns11 也具有 VSR 功能的证据。 Pns10 位于细胞质中，而 Pns11 则位于细胞核和叶绿体中。 Pns11 具有两个二分核定位信号 (NLS)，这是核定位和叶绿体定位所必需的。 NLS 也是 Pns11 沉默活动所必需的。这是首次报道病毒编码的多个 VSR 位于不同的亚细胞区室中，并且病毒蛋白可以靶向细胞核和叶绿体。这些发现对于研究病毒-宿主相互作用中 VSR 和其他病毒蛋白的亚细胞靶向可能具有广泛的意义。
RNA silencing is a potent antiviral mechanism in plants and animals. As a counter-defense, many viruses studied to date encode one or more viral suppressors of RNA silencing (VSR). In the latter case, how different VSRs encoded by a virus function in silencing remains to be fully understood. We previously showed that the nonstructural protein Pns10 of a Phytoreovirus,Rice dwarf virus(RDV), functions as a VSR. Here we present evidence that another nonstructural protein, Pns11, also functions as a VSR. While Pns10 was localized in the cytoplasm, Pns11 was localized both in the nucleus and chloroplasts. Pns11 has two bipartite nuclear localization signals (NLSs), which were required for nuclear as well as chloroplastic localization. The NLSs were also required for the silencing activities of Pns11. This is the first report that multiple VSRs encoded by a virus are localized in different subcellular compartments, and that a viral protein can be targeted to both the nucleus and chloroplast. These findings may have broad significance in studying the subcellular targeting of VSRs and other viral proteins in viral-host interactions.