A Retentive Memory of Tetrachloroethene Respiration in Sulfurospirillum halorespirans - involved Proteins and a possible link to Acetylation of a Two-Component Regulatory System.
A Retentive Memory of Tetrachloroethene Respiration in Sulfurospirillum halorespirans - involved Proteins and a possible link to Acetylation of a Two-Component Regulatory System.
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DOI：
10.1016/j.jprot.2018.03.030
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发表时间：
2018-06
影响因子：
3.3
通讯作者：
Dominique Türkowsky;Jens Esken;Tobias Goris;T. Schubert;G. Diekert;N. Jehmlich;M. von Bergen
中科院分区：
文献类型：
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作者：
Dominique Türkowsky;Jens Esken;Tobias Goris;T. Schubert;G. Diekert;N. Jehmlich;M. von Bergen
有机卤化物呼吸(OHR)包括卤代有机化合物的还原脱卤化，受独特的记忆效应和多食硫杆菌相关基因的长期转录下调的影响。在没有四氯乙烯(PCE)的情况下，基因表达在大约100代中缓慢停止。然而，这一调控过程的分子机制尚不清楚。我们在这里表明，在没有氯化乙烯的情况下，长时间培养的卤化硫杆菌也经历了同样的调节。此外，我们还比较了OFS的蛋白质组。在PCE存在的情况下培养的细胞与以硝酸盐为唯一电子受体的长期和短期培养的细胞的卤素呼吸。重要的OHR相关蛋白，以前未确定的INS。多基因包括一个组氨酸激酶、一个可能的喹酚脱氢酶膜蛋白和一个PCE诱导的孔蛋白。由于对于一些调节蛋白来说，赖氨酸乙酰化对活性的翻译后调节是已知的，我们也分析了乙酰组OFS。发现32%的蛋白质组在至少一种条件下是乙酰化的。这些数据表明，在没有PCE的情况下，在硝酸盐短期培养过程中，反应调节器和组氨酸激酶最可能参与PCE呼吸的诱导，高度乙酰化。有意义的是，到目前为止，在第二个物种中发现了唯一的有机卤化物呼吸长期下调的现象，这表明这种调节现象的分布更广泛。一种改进的蛋白质提取方法可以鉴定硫代螺旋藻中最可能参与OHR转录调控的蛋白质。我们的数据表明，在缺乏底物的情况下，调节蛋白的乙酰化参与了代谢酶的这种极端的、持续的待机模式。这一首次发表的变形杆菌乙酰组可能有助于研究这一分支的其他生态或医学上重要的物种。
Organohalide respiration (OHR), comprising the reductive dehalogenation of halogenated organic compounds, is subject to a unique memory effect and long-term transcriptional downregulation of the involved genes inSulfurospirillum multivorans. Gene expression ceases slowly over approximately 100 generations in the absence of tetrachloroethene (PCE). However, the molecular mechanisms of this regulation process are not understood. We show here thatSulfurospirillum halorespiransundergoes the same type of regulation when cultivated without chlorinated ethenes for a long period of time. In addition, we compared the proteomes ofS. halorespiranscells cultivated in the presence of PCE with those of cells long- and short-term cultivated with nitrate as the sole electron acceptor. Important OHR-related proteins previously unidentified inS. multivoransinclude a histidine kinase, a putative quinol dehydrogenase membrane protein, and a PCE-induced porin. Since for some regulatory proteins a posttranslational regulation of activity by lysine acetylations is known, we also analyzed the acetylome ofS. halorespirans, revealing that 32% of the proteome was acetylated in at least one condition. The data indicate that the response regulator and the histidine kinase of a two-component system most probably involved in induction of PCE respiration are highly acetylated during short-term cultivation with nitrate in the absence of PCE.SignificanceThe so far unique long-term downregulation of organohalide respiration is now identified in a second species suggesting a broader distribution of this regulatory phenomenon. An improved protein extraction method allowed the identification of proteins most probably involved in transcriptional regulation of OHR inSulfurospirillumspp. Our data indicate that acetylations of regulatory proteins are involved in this extreme, sustained standby-mode of metabolic enzymes in the absence of a substrate. This first published acetylome of Epsilonproteobacteria might help to study other ecologically or medically important species of this clade.