Effects of electrical stimulation and seminal plasma on the motility of mouse sperm
Effects of electrical stimulation and seminal plasma on the motility of mouse sperm
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DOI：
10.1071/rd02050
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发表时间：
2002-01-01
影响因子：
1.9
通讯作者：
Trounson, AO
中科院分区：
文献类型：
--
作者：
Tecirlioglu, RT;Lacham-Kaplan, O;Trounson, AO
通过对C57BLx CBA和C57BL/6J小鼠的精子运动参数、受精情况和胚胎发育的研究，探讨了电流和精浆对C57BLx CBA和C57BL/6J小鼠附睾和射精精子的影响。采用四电极直肠探头，施加计算机产生的正弦交流电(0.25V~3.0V，频率50赫兹)，对1、2、3周龄的S分别给予1、2、3次静息时间，持续4min，获得精液。将同一组小鼠的附睾精子分别置于有机玻璃小室和含精浆的培养液中培养2 h，结果表明，电流作用与含精浆的培养液培养2 h相比，差异显著(P<0.05)。
The effects of electric current (in vivo and in vitro) and seminal plasma on epididymal and ejaculated sperm obtained from C57BL x CBA and C57BL/6J mice were investigated by studying motility parameters, fertilization and embryo development. Electroejaculates were obtained by applying a series of computer-generated sinusoidal alternating currents (0.25-3.0 V at 50 Hz) delivered for 1, 2 and 3 s with 1-s rest periods using a four-electrode rectal probe for 4 min. Epididymal sperm obtained from the same mice were either subjected to electric current in vitro in a Plexiglass chamber or incubated in a medium containing seminal plasma for 2 h. In vitro electric current application and incubation in a medium containing seminal plasma significantly (P