Overexpression of chloride channel-3 (ClC-3) is associated with human cervical carcinoma development and prognosis
Overexpression of chloride channel-3 (ClC-3) is associated with human cervical carcinoma development and prognosis
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DOI：
10.1186/s12935-018-0721-x
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发表时间：
2019-01-08
影响因子：
5.8
通讯作者：
Wang, Gang
中科院分区：
文献类型：
--
作者：
Guan, Yu-tao;Xie, Yong;Wang, Gang
背景宫颈癌是妇科的主要恶性肿瘤之一，在世界范围内引起癌症相关的死亡，其潜在的发病机制和进展仍处于研究阶段。ClC-3可能是恶性肿瘤侵袭转移的重要促进因子。本研究探讨ClC-3在宫颈癌组织中的表达及其临床意义，试图阐明ClC-3在肿瘤恶性行为、发展和预后中的可能作用。采用实时荧光定量PCR和免疫印迹法检测165例新鲜人宫颈组织标本和4株人宫颈细胞系中ClC-3 mRNA和蛋白的表达水平。结果在正常宫颈组织、宫颈癌前病变（LSIL、HSIL）及宫颈癌组织中，ClC-3 mRNA及蛋白表达水平均显著增高（P
BackgroundCervical carcinoma is a major gynecological cancer and causes cancer-related deaths in worldwide, the latent pathogenesis and progress of cervical cancer is still under research. ClC-3 may be an important promoter for aggressive metastasis of malignant tumors. In this research, we explore the ClC-3 expression in cervical carcinoma and its underlying clinical significance, trying to illuminate ClC-3 probable function in the neoplasm malignant behavior, development and prognosis.MethodsParaffin-embedded cervical (n=168) and lymph node (n=100) tissue specimens were analysed by immunohistochemistry. Fresh human cervical tissue specimens (n=165) and four human cervical cell lines were tested for ClC-3 mRNA and protein expression levels by quantitative real-time PCR and western blotting. The relationship between the expression levels of ClC-3, the pathological characteristics of the carcinoma, and the clinical prognosis were statistically analysed.ResultsIn normal and precancerous (LSIL, HSIL) cervical tissues as well as cervical carcinoma tissues, both ClC-3 mRNA and protein expression levels increased significantly (p