Mapping of Brain Activity by Automated Volume Analysis of Immediate Early Genes.
Mapping of Brain Activity by Automated Volume Analysis of Immediate Early Genes.
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DOI：
10.1016/j.cell.2016.05.007
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发表时间：
2016-06-16
期刊：
影响因子：
64.5
通讯作者：
Tessier-Lavigne M
中科院分区：
文献类型：
--
作者：
Renier N;Adams EL;Kirst C;Wu Z;Azevedo R;Kohl J;Autry AE;Kadiri L;Umadevi Venkataraju K;Zhou Y;Wang VX;Tang CY;Olsen O;Dulac C;Osten P;Tessier-Lavigne M
了解神经信息在生理和病理状态下是如何处理的，将受益于对整个大脑中所有神经元活动的精确检测、定位和量化，而迄今为止，这在哺乳动物大脑中尚未实现。我们引入了一种以细胞分辨率高速采集大脑活动的管道，通过使用免疫染色和光片荧光成像分析立即早期基因表达，然后通过我们称为 ClearMap 的开源软件程序对活动进行自动绘图和分析。我们首先通过分析响应氟哌啶醇激活的大脑区域来验证该流程。接下来，我们报告在主动探索过程中胡须诱发的感觉处理下游的新皮质区域。最后，我们将活动映射与轴突追踪相结合，以发现在养育行为期间差异激活的新大脑区域。该管道广泛适用于不同的实验范式，包括不易获得转基因活动报告基因的动物物种。
Understanding how neural information is processed in physiological and pathological states would benefit from precise detection, localization and quantification of the activity of all neurons across the entire brain, which has not to date been achieved in the mammalian brain. We introduce a pipeline for high speed acquisition of brain activity at cellular resolution through profiling immediate early gene expression using immunostaining and light-sheet fluorescence imaging, followed by automated mapping and analysis of activity by an open-source software program we term ClearMap. We validate the pipeline first by analysis of brain regions activated in response to Haloperidol. Next, we report new cortical regions downstream of whisker-evoked sensory processing during active exploration. Lastly, we combine activity mapping with axon tracing to uncover new brain regions differentially activated during parenting behavior. This pipeline is widely applicable to different experimental paradigms, including animal species for which transgenic activity reporters are not readily available.