Simultaneous removal of parathion and methyl parathion by genetically engineered Escherichia coli in a biofilter treating polluted air
Simultaneous removal of parathion and methyl parathion by genetically engineered Escherichia coli in a biofilter treating polluted air
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DOI：
10.1504/ijep.2011.039080
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发表时间：
2011-03
影响因子：
0.7
通讯作者：
Lin Li;Chao Yang;Yaqim He;C. Qiao;Jun-xin Liu
中科院分区：
文献类型：
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作者：
Lin Li;Chao Yang;Yaqim He;C. Qiao;Jun-xin Liu
用基因工程大肠杆菌菌株接种的生物过滤器成功地用于处理含有对硫磷和甲基对硫磷（MP）混合物的气流。在整个运行期间，对对硫磷的平均去除率为95.2%，对甲基磷的平均去除率为98.6%。与传统的生物过滤器相比，接种了工程大肠杆菌BL21的生物过滤器的效果要高得多，特别是在初始阶段。对硫磷和MP可在一个生物滤池内同时处理。代谢物分析表明，对硫磷和MP被水解为对硝基酚（PNP）。此外，在生物过滤器运行过程中检测硝酸盐和硫酸盐。对生物过滤器的性能和微生物群落进行了评价和表征。
A biofilter inoculated with a genetically engineered Escherichia coli strain was successfully applied to treat air streams containing a mixture of parathion and methyl parathion (MP). Average removal efficiencies of 95.2% for parathion and 98.6% for MP were obtained during the overall operation period. Compared with conventional biofilters, the biofilter inoculated with the engineered E. coli BL21 was far more effective, especially in the initial stages. Parathion and MP could be treated simultaneously within one biofilter. Metabolite analysis revealed that parathion and MP were hydrolysed to p-Nitrophenol (PNP). Additionally, nitrate and sulphate were detected during the biofilter operation. The biofilter performance and microbial community were also assessed and characterised.