Engineering luminescent quantum dots for In vivo molecular and cellular imaging
Engineering luminescent quantum dots for In vivo molecular and cellular imaging
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DOI：
10.1007/s10439-005-9000-9
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发表时间：
2006-01-01
影响因子：
3.8
通讯作者：
Nie, SM
中科院分区：
文献类型：
--
作者：
Smith, AM;Ruan, G;Nie, SM
半导体量子点是一种发光纳米粒子，作为一种新型光学成像造影剂正在得到广泛的发展。它们的新特性，如光学可调性、改进的光稳定性和多色光发射，为以前所未有的灵敏度和空间分辨率成像活细胞和体内动物模型开辟了新的机会。结合在分子水平上定制粒子表面的生物分子工程策略，生物共轭量子点探针非常适合成像活细胞中的单分子动力学，监测特定细胞内位置的蛋白质-蛋白质相互作用，以及检测深层组织中的病变部位和器官。在本文中，我们描述了制备高质量量子点和将量子点偶联到生物分子配体的工程原理。我们还讨论了利用量子点进行体内分子和细胞成像的最新进展。
Semiconductor quantum dots are luminescent nanoparticles that are under intensive development for use as a new class of optical imaging contrast agents. Their novel properties such as optical tunability, improved photostability, and multicolor light emission have opened new opportunities for imaging living cells and in vivo animal models at unprecedented sensitivity and spatial resolution. Combined with biomolecular engineering strategies for tailoring the particle surfaces at the molecular level, bioconjugated quantum dot probes are well suited for imaging single-molecule dynamics in living cells, for monitoring protein-protein interactions within specific intracellular locations, and for detecting diseased sites and organs in deep tissue. In this article, we describe the engineering principles for preparing high-quality quantum dots and for conjugating the dots to biomolecular ligands. We also discuss recent advances in using quantum dots for in vivo molecular and cellular imaging.