ENZYME ENTRAPMENT IN LIPOSOMES
ENZYME ENTRAPMENT IN LIPOSOMES
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DOI：
10.1016/0014-5793(71)80109-6
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发表时间：
1971-01-01
期刊：
影响因子：
3.5
通讯作者：
RYMAN, BE
中科院分区：
文献类型：
--
作者：
GREGORIA.G;LEATHWOO.PD;RYMAN, BE
对于患有一种或多种组织中缺乏特定酶活性的各种疾病的患者，已经在几种情况下尝试了酶替代疗法，即直接向患者施用旨在去除不需要的积累产物[IS]的酶。然而，除了外源蛋白可能引起的免疫反应外，还有一些问题与不希望在循环中有某些酶和将给定的蛋白导向特定的组织有关，我们认为，上述的一些困难可以通过将蛋白包埋在脂质体（脂质小球）中来避免。当允许磷脂在水性介质中溶胀并变成水合液晶时形成脂质体。当适当分散时，它们由一系列同心双层组成，这些双层与水室交替，其中可以捕获水溶性物质[6，7]。本文报道了尼日尔曲霉淀粉葡萄糖苷酶（EC 3.2. 1.3.）和白蛋白转化为脂质体。在此截留调查中的淀粉葡糖苷酶的选择反映了我们的兴趣，在糖原储存疾病，而商业可用性的r3 r I-白蛋白提供了一个容易检测的蛋白质，类似的分子大小和电荷的淀粉葡糖苷酶。
Enzyme replacement therapy for patients with various disorders in which a specific enzyme activity is absent from one or more tissues, has been attempted in several instances by direct administration to the patient of an enzyme designed to remove undesirable accumulation products [IS]. However, apart from the possible immunological response that could arise from the foreign protein, there are also the problems related to undesirability of having certain enzymes in the circulation and of directing the given protein to a particular tissue.We thought that some of the difficulties mentioned could be circumvented by entrapping proteins into liposomes (lipid spherules). Liposomes are formed when phospholipids are allowed to swell in aqueous media and become hydrated liquid crystals. These, when suitably dispersed, consist of a series of concentric bilayers which alternate with aqueous compartments in which can be entrapped water-soluble substances [6, 7]. The present paper describes the entrapment ofAsper-giZlus niger amyloglucosidase (EC 3.2. 1.3.) and albumin into liposomes. The choice of the amyloglucosidase in this entrapment investigation reflects our interest in the glycogen storage diseases, while the commercial availability of r3r I-albumin has provided a readily detectable protein, similar in both molecular size and charge to the amyloglucosidase.