Characterization of p-Coumaric acid-induced soluble and cell wall-bound phenolic metabolites in relation to disease resistance to Xanthomonas campestris pv. campestris in Chinese cabbage
Characterization of p-Coumaric acid-induced soluble and cell wall-bound phenolic metabolites in relation to disease resistance to Xanthomonas campestris pv. campestris in Chinese cabbage
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DOI：
10.1016/j.plaphy.2018.02.012
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发表时间：
2018-04-01
影响因子：
6.5
通讯作者：
Kim, Tae-Hwan
中科院分区：
文献类型：
--
作者：
Islam, Md Tabibul;Lee, Bok-Rye;Kim, Tae-Hwan
探讨对香豆酸（pCA）诱导的酚类代谢物与油菜黄单胞菌（Xanthomonas campestris pv）抗病的关系。研究了可溶性和细胞壁结合的类黄酮和羟基肉桂酸化合物在接种Xcc后对pCA预处理或4-香豆酸-辅酶a连接酶抑制剂3,4-（亚甲基二氧基）肉桂酸（MDCA）的反应，并对结果数据进行了对中国白菜（Brassica rapa var. pekinensis） Xcc敏感性的解释。在接种Xcc后12天，疾病症状的发展可以通过坏死病灶来区分，并以脂质过氧化增强为特征。总的来说，pCA对羟基肉桂酸和类黄酮的积累起着积极的刺激作用，而MDCA起着消极的调节作用。pCA预处理导致xcc接种叶片中特异性羟基肉桂酸、pCA、阿魏酸（FA）和辛酸（SiA）以可溶性和细胞壁结合形式积累，而MDCA预处理以剂量依赖的方式减少积累。两种黄酮类化合物表没食子儿茶素（EGC）和表没食子儿茶素没食子酸酯（EGCG）对pCA和MDCA预处理的反应相似。这些结果表明，pca预处理叶片中较低的疾病症状发展与羟基肉桂酸和类黄酮的积累较高相关，反之亦然。
To characterize the p-coumaric acid (pCA)-induced phenolic metabolites in relation to the disease resistance against Xanthomonas campestris pv. campestris (Xcc.), the responses of soluble- and cell wall-bound flavonoid and hydroxycinnamic acids compounds to the pretreatment of pCA or the inhibitor of the 4-coumarate-CoA ligase, 3,4-(methylenedioxy) cinnamic acid (MDCA), following Xcc inoculation were assessed, and the resulting data were interpreted with regard to susceptibility to Xcc in Chinese cabbage (Brassica rapa var. pekinensis). At 12 days post-inoculation (DPI) with Xcc, disease symptom development could be distinguished by necrotic lesions, and characterized by an enhanced lipid peroxidation. Overall, pCA acts as a positive stimulus for an accumulation of hydroxycinnamic acids and flavonoids, while MDCA acts as a negative regulator. Pretreatment with pCA resulted in an accumulation of specific hydroxycinnamic acids, pCA, ferulic acid (FA), and sinapic acid (SiA) in both soluble and cell wall-bound forms in Xcc-inoculated leaves, while MDCA pretreatment decreased accumulation in a dose-dependent manner. Two flavonoid compounds, epigallocatechin (EGC) and epigallocatechin gallate (EGCG), showed a similar response to pCA and MDCA pretreatments. These results indicate that a lower disease symptom development in pCA-pretreated leaves was associated with a higher accumulation of hydroxycinnamic acids and flavonoids, and vice-versa in MDCA- and non-pretreated (control) leaves.