Experimental animal models for COPD: a methodological review.
Experimental animal models for COPD: a methodological review.
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DOI：
10.1186/s12971-017-0130-2
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发表时间：
2017
影响因子：
3.7
通讯作者：
Kianmeher M
中科院分区：
文献类型：
--
作者：
Ghorani V;Boskabady MH;Khazdair MR;Kianmeher M
慢性阻塞性肺疾病（COPD）是一种进行性疾病，其使呼吸困难，并且特征在于从慢性炎症到组织蛋白水解的病理状况。关于COPD患者研究的伦理问题，使用COPD动物模型是不可避免的。动物模型提高了我们对COPD生理学、病理生理学和治疗的基本机制的认识。虽然这些模型只能模拟疾病的一些特征，但它们对于进一步研究人类COPD的机制是有价值的。我们在Google Scholar、PubMed和ScienceDirect数据库中检索了截至2015年11月发表的英文文献。为此，我们使用了5个COPD关键词，3个动物模型关键词，4个暴露方法关键词，3个病理生理学变化关键词和3个生物标志物关键词。151项研究被认为有资格纳入本次审查。根据综述的文献，COPD动物模型主要采用小鼠、豚鼠和大鼠。在大多数研究中，该模型通过暴露于香烟烟雾（CS）、气管内脂多糖（LPS）和鼻内弹性蛋白酶诱导。在不同的研究中，诱导剂的时间过程和剂量存在差异。在大多数研究中，主要测量的参数是肺病理学数据和肺炎症（炎症细胞和炎症介质），只有少数研究是气管反应性（TR）。本文综述了COPD动物模型的各种诱导方法、所用动物（主要是小鼠、豚鼠和大鼠）及测定参数。本文对COPD相关研究中选择合适的动物、诱导方法和测定参数有一定的参考价值。
Chronic obstructive pulmonary disease (COPD) is a progressive disorder that makes the breathing difficult and is characterized by pathological conditions ranging from chronic inflammation to tissue proteolysis. With regard to ethical issues related to the studies on patients with COPD, the use of animal models of COPD is inevitable. Animal models improve our knowledge about the basic mechanisms underlying COPD physiology, pathophysiology and treatment. Although these models are only able to mimic some of the features of the disease, they are valuable for further investigation of mechanisms involved in human COPD. We searched the literature available in Google Scholar, PubMed and ScienceDirect databases for English articles published until November 2015. For this purpose, we used 5 keywords for COPD, 3 for animal models, 4 for exposure methods, 3 for pathophysiological changes and 3 for biomarkers. One hundred and fifty-one studies were considered eligible for inclusion in this review. According to the reviewed articles, animal models of COPD are mainly induced in mice, guinea pigs and rats. In most of the studies, this model was induced by exposure to cigarette smoke (CS), intra-tracheal lipopolysaccharide (LPS) and intranasal elastase. There were variations in time course and dose of inducers used in different studies. The main measured parameters were lung pathological data and lung inflammation (both inflammatory cells and inflammatory mediators) in most of the studies and tracheal responsiveness (TR) in only few studies. The present review provides various methods used for induction of animal models of COPD, different animals used (mainly mice, guinea pigs and rats) and measured parameters. The information provided in this review is valuable for choosing appropriate animal, method of induction and selecting parameters to be measured in studies concerning COPD.
影响因子：
2.5
作者：
Rafiq M;Viswanatha GL;Suryakanth DA;Azeemuddin M;Jagadeesh M;Dhanush K;Patki PS
通讯作者：
Patki PS