Quantitative analysis of vascular colonisation and angio-conduction in porous silicon-substituted hydroxyapatite with various pore shapes in a chick chorioallantoic membrane (CAM) model
Quantitative analysis of vascular colonisation and angio-conduction in porous silicon-substituted hydroxyapatite with various pore shapes in a chick chorioallantoic membrane (CAM) model
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DOI：
10.1016/j.actbio.2016.04.039
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发表时间：
2016-07-01
影响因子：
9.7
通讯作者：
Champion, Eric
中科院分区：
文献类型：
--
作者：
Magnaudeix, Amandine;Usseglio, Julie;Champion, Eric
大缺损骨填充支架的开发需要了解血管生成和血管引导，这是骨形成和愈合的关键过程。关于支架支持血管引导的能力的研究很少，这是非常重要的，因为它关系到血管网络的质量和分散。本文报道了多孔硅取代羟基磷灰石（SiHA）生物陶瓷的血管化分析，以及孔隙形状对血管引导的影响，采用一种方便的卵生模型，即鸡胚绒毛膜尿囊膜（CAM）试验。血管植入物的图像分析评估了两种不同尺度（整个陶瓷和单独孔隙）的血管密度、分形维数和血管直径，并对具有各种截面几何形状孔隙（圆形、方形、菱形、三角形和星形）的SiHA模型陶瓷进行了分析。SiHA是一种生物相容性材料，允许血管在其表面传导。孔隙的存在不影响血管生成相关参数（树形、分形维数），但孔隙几何形状影响血管引导和血管传导电位（向孔隙聚集的血管直径和数量）。测量的孔横截面角度调节了向孔聚集的血管的数量和直径，其中三角形孔显得特别有趣。这一结果将用于塑造具有特定多孔结构的陶瓷支架，以促进血管定植和骨整合。本文建立了一种简便有效的方法，利用鸡胚绒毛尿囊膜（CAM）测定支架内血管生成和血管传导。该方法是对常规细胞培养试验的补充，可在一定程度上替代体内实验。将其应用于具有不同孔隙形状的硅取代羟基磷灰石多孔生物陶瓷。这种材料被发现具有生物相容性，允许血管在其表面传导。毛细孔的存在不影响血管的生成，但毛细孔的形状影响血管的引导和血管传导电位。为了促进支架的血管定植和骨整合并提高其性能，具有三角形横截面的孔隙对进一步设计支架具有特别的吸引力。(C) 2016材料学报Elsevier Ltd.出版。版权所有。
The development of scaffolds for bone filling of large defects requires an understanding of angiogenesis and vascular guidance, which are crucial processes for bone formation and healing. There are few investigations on the ability of a scaffold to support blood vessel guidance and it this is of great importance because it relates to the quality and dispersion of the blood vessel network. This work reports an analysis of vascularisation of porous silicon-substituted hydroxyapatite (SiHA) bioceramics and the effects of pore shape on vascular guidance using an expedient ex ovo model, the chick embryo chorioallantoic membrane (CAM) assay. Image analysis of vascularised implants assessed the vascular density, fractal dimension and diameter of blood vessels at two different scales (the whole ceramic and pores alone) and was performed on model SiHA ceramics harbouring pores of various cross-sectional geometries (circles, square, rhombus, triangles and stars). SiHA is a biocompatible material which allows the conduction of blood vessels on its surface. The presence of pores did not influence angiogenesis related-parameters (arborisation, fractal dimension) but pore geometry affected the blood vessel guidance and angioconductive potential (diameter and number of the blood vessels converging toward the pores). The measured angles of pore cross-section modulated the number and diameter of blood vessels converging to pores, with triangular pores appearing of particular interest. This result will be used for shaping ceramic scaffolds with specific porous architecture to promote vascular colonisation and osteointegration.Statement of SignificanceAn expedient and efficient method, using chick embryo chorioallantoic membrane (CAM) assays, has been set up to characterise quantitatively the angiogenesis and the vascular conduction in scaffolds. This approach complements the usual cell culture assays and could replace to a certain extent in vivo experiments. It was applied to silicon-substituted hydroxyapatite porous bioceramics with various pore shapes. The material was found to be biocompatible, allowing the conduction of blood vessels on its surface. The presence of pores does not influence the angiogenesis but the pore shape affects the blood vessel guidance and angio-conductive potential. Pores with triangular cross-section appear particularly attractive for the further design of scaffolds in order to promote their vascular colonisation and osteointegration and improve their performances. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.