Downregulation of type I collagen expression in the Achilles tendon by dexamethasone: a controlled laboratory study
Downregulation of type I collagen expression in the Achilles tendon by dexamethasone: a controlled laboratory study
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地塞米松下调跟腱中 I 型胶原蛋白的表达：一项对照实验室研究。
DOI：
10.1186/s13018-020-01602-z
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发表时间：
2020-02-24
影响因子：
2.6
通讯作者：
Tang, Kanglai
中科院分区：
文献类型：
--
作者：
Ge, Zilu;Tang, Hong;Tang, Kanglai
背景：自发性跟腱断裂与长期使用地塞米松（Dex）有关的报道。然而，很少有研究探讨了潜在的机制。本研究的目的是评估口服地塞米松对人类和大鼠I型胶原蛋白的影响及其与肌腱rupture.Methods的关联：首先，从患者谁接受长期地塞米松治疗的跟腱，另外6个正常肌腱组织学评价收获。其次，8周龄大鼠（n=72）被随机分配到地塞米松组或对照组。在3周和5周后，在机械、组织学和分子水平上研究I型胶原。从正常人和大鼠肌腱分离的Tenocytes被用来调查Dex对细胞scale.Results的影响：人类和大鼠肌腱组织的组织学分析显示，与对照组相比，在Dex组的I型胶原的不规则，无序排列。此外，在Dex+组中，与Dex-组相比，人和大鼠肌腱细胞中的I型胶原表达降低。5周后，Dex组肌腱的机械强度（68.87 ± 11.07 N）显著低于对照组（81.46 ± 7.62 N，P=0.013）。5周后，Dex组的肌腱短于对照组（10.71 ± 0.34mm（2），1.44 ± 0.22mm（2），P=0.050，2.74 ± 0.34mm（2），P
Background: Spontaneous Achilles tendon rupture associated with long-term dexamethasone (Dex) use has been reported. However, few studies have investigated the potential mechanism. The aim of this study was to evaluate the effects of oral Dex on type I collagen in humans and rats and its association with tendon rupture.Methods: First, six Achilles tendons from patients who received long-term Dex treatment, and another six normal tendons were harvested for histological evaluation. Secondly, 8-week-old rats (n=72) were randomly assigned to a Dex group or a control group. Type I collagen was studied at the mechanical, histological, and molecular levels after 3 and 5weeks. Tenocytes isolated from normal human and rat tendon were used to investigate the effect of Dex on cellular scale.Results: Histological analysis of human and rat tendon tissue revealed an irregular, disordered arrangement of type I collagen in the Dex group compared with the control group. In addition, In the Dex+ group, type I collagen expression decreased in comparison with the Dex-group in both human and rat tenocytes. The mechanical strength of tendons was significantly reduced in the Dex group (68.8711.07N) in comparison with the control group (81.46 +/- 7.62N, P=0.013) after 5weeks. Tendons in the Dex group were shorter with smaller cross-sectional areas (10.71 +/- 0.34mm(2), 1.44 +/- 0.22mm(2), respectively) after 5weeks than those in the control group (11.13 +/- 0.50mm(2), P=0.050, 2.74 +/- 0.34mm(2), P