Preserved extracellular matrix components and retained biological activity in decellularized porcine mesothelium
Preserved extracellular matrix components and retained biological activity in decellularized porcine mesothelium
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DOI：
10.1016/j.biomaterials.2010.05.026
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发表时间：
2010-09-01
期刊：
影响因子：
14
通讯作者：
Vacanti, Joseph R.
中科院分区：
文献类型：
--
作者：
Hoganson, David M.;Owens, Gwen E.;Vacanti, Joseph R.
腹膜和胸膜等间皮组织有一层薄而强的细胞外基质，支持间皮细胞快速愈合。对脱细胞猪间皮的强度、基质组成和生物活性进行了表征。材料的抗拉强度为40.65+/-21.65N/cm。材料中含有细胞外基质蛋白、IV型胶原、纤维连接蛋白、层粘连蛋白和糖胺聚糖。细胞外基质中固有的细胞因子被保留下来。血管内皮生长因子、成纤维细胞生长因子和转化生长因子-β在脱细胞间皮中的表达水平高于脱细胞的小肠黏膜下层。脱细胞间皮细胞还刺激人成纤维细胞产生比生长在组织培养塑料上的成纤维细胞更多的血管内皮生长因子。脱细胞间皮是一种兼具强度和生物活性的片状材料，在外科修复和再生医学中具有潜在的应用前景。(C)2010爱思唯尔有限公司。保留所有权利。
Mesothelium tissues such as peritoneum and pleura have a thin and strong layer of extracellular matrix that supports mesothelial cells capable of rapid healing. Decellularized porcine mesothelium was characterized for strength, composition of the matrix and biological activity. The tensile strength of the material was 40.65 +/- 21.65 N/cm. Extracellular matrix proteins collagen IV, fibronectin, and laminin as well as glycosaminoglycans were present in the material. Cytokines inherent in the extracellular matrix were preserved. Vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and transforming growth factor beta (TGF-beta) were retained and the levels of VEGF and TGF-beta in the decellularized mesothelium were higher than those found in decellularized small intestinal submucosa (SIS). The decellularized mesothelium also stimulated human fibroblasts to produce more VEGF than fibroblasts grown on tissue culture plastic. Decellularized mesothelium is a sheet material with a combination of strength and biological activity that may have many potential applications in surgical repair and regenerative medicine. (C) 2010 Elsevier Ltd. All rights reserved.