POLARIZATION OF MITOCHONDRIA IN THE UNFERTILIZED MOUSE OOCYTE
POLARIZATION OF MITOCHONDRIA IN THE UNFERTILIZED MOUSE OOCYTE
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DOI：
10.1002/dvg.1020160108
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发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
CALARCO, PG
中科院分区：
文献类型：
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作者：
CALARCO, PG
一个未成熟的卵母细胞成熟为一个能够受精的卵母细胞涉及染色体和细胞器紧密编排的运动。用共聚焦激光扫描显微镜（CLSM）研究了活体小鼠卵母细胞成熟过程中线粒体的定位。线粒体用罗丹明123或Mitotracker （Molecular Probes, Eugene, or）标记，两者都是细胞渗透并积聚在线粒体中；用吖啶橙标记染色质。成熟前卵母细胞呈放射状对称，无明显极性；完全成熟的卵母细胞表现出明显的极性，以中期II纺锤体在皮层中的位置为标志。CLSM揭示了线粒体分布的几个有趣特征：1)在中期II纺锤体一侧约30-45度可见皮质线粒体团块，标志着极体I挤压区。2)在成熟卵母细胞的中心区域周围，经常发现大的线粒体灶（7-14 μ M），而中心区域的线粒体往往明显较少。3)皮层和GV附近的小线粒体灶（3 μ M）表明有几个卵母细胞未成熟。4)非纺锤体相关线粒体在成熟卵母细胞中分布不均匀，而是集中在含有中期纺锤体的半球。5)线粒体半球的远端边缘在皮层处界限分明。这些发现将有助于我们了解哺乳动物卵母细胞成熟过程中的细胞器定位，并可能对不育的可能原因和着床前发育的早期事件提供见解。(C) 1995 Wiley-Liss, Inc。
Maturation of an immature oocyte into one capable of being fertilized involves tightly choreographed movements of chromosomes and organelles. The localization of mitochondria during maturation was studied in live mouse oocytes by confocal laser scanning microscopy (CLSM). Mitochondria were labeled with rhodamine 123 or Mitotracker (Molecular Probes, Eugene, OR) both of which are cell permeant and accumulate in mitochondria; acridine orange was used to mark chromatin. Prior to maturation, oocytes appeared to be radially symmetrical with no evident polarity; fully mature oocytes exhibited obvious polarity marked by the position of the metaphase II spindle in the cortex. CLSM revealed several interesting features of mitochondrial distribution: 1) A cortical clump of mitochondria was seen approximately 30-45 degrees to one side of the metaphase II spindle and marked the region of polar body I extrusion. 2) Large foci of mitochondria (7-14 mu M) were frequently found around the central region of the mature oocyte, while the central region often exhibited markedly fewer mitochondria. 3) Small mitochondrial foci (3 mu M) in the cortex and near the GV characterized several oocytes which failed to mature. 4) Non-spindle-associated mitochondria were not uniformly distributed in the mature oocyte but were concentrated in the hemisphere containing the metaphase II spindle. 5) The distal margins of this mitochondrial hemisphere were sharply demarcated at the cortex. These findings should help us understand organelle localization during mammalian oocyte maturation, and may give insights into possible causes of infertility and into early events of preimplantation development. (C) 1995 Wiley-Liss, Inc.