Changes in the epigenome and transcriptome of rice in response to Magnaporthe oryzae infection
Changes in the epigenome and transcriptome of rice in response to Magnaporthe oryzae infection
复制标题
稻瘟病菌感染引起的水稻表观基因组和转录组的变化
DOI：
10.1016/j.cj.2020.10.002
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发表时间：
2021-08-01
期刊：
影响因子：
6.6
通讯作者：
Wang, Haifeng
中科院分区：
文献类型：
--
作者：
Cui, Na;Chen, Xiaofeng;Wang, Haifeng
DNA甲基化参与调控植物编码区和非编码区的表达。为了研究DNA甲基化与病原菌侵染之间的关系，我们利用全基因组测序技术研究了稻瘟病菌侵染水稻后，水稻体内DNA甲基化的时间变化。与之前在拟南芥中的发现相反，稻瘟病侵染后，水稻中的全球DNA甲基化水平略有增加。我们确定了超过38,000个差异甲基化区域(DMR)，并且高甲基化的DMR的数量远远多于低甲基化的DMR。大多数DMR位于转座元件区域。利用转录组分析，我们在感染后1、3和5天鉴定了8830个差异表达基因(Deg)。超过三分之一的DEG，其中大多数是CHH型DMR，与DMR有关。对CHH DMR-DEG的功能分析表明，它们参与了许多重要的生物学过程，包括细胞通讯和对外部刺激的反应。许多NBS-LRR家族基因的转录受到DNA甲基化变化的影响，这表明DNA甲基化在水稻对稻瘟病菌侵染的反应中起着至关重要的作用。更广泛地说，这里提出的DNA甲基化分析揭示了表观基因组参与植物抵御真菌病原体的防御。(C)2021年中国作物科学学会和中国科学院作物科学研究所。爱思唯尔B.V.代表科爱通信有限公司制作和主办。
DNA methylation participates in regulating the expression of coding and non-coding regions in plants. To investigate the association between DNA methylation and pathogen infection, we used whole-genome bisulfite sequencing to survey temporal DNA methylation changes in rice after infection with the rice blast fungus Magnaporthe oryzae. In contrast to previous findings in Arabidopsis, global DNA methylation levels in rice increased slightly after rice blast infection. We identified over 38,000 differentially methylated regions (DMRs), and hypermethylated DMRs far outnumbered hypomethylated DMRs. Most DMRs were located in transposable element regions. Using transcriptome analysis, we identified 8830 differentially expressed genes (DEGs) after 1, 3, and 5 days of infection. Over one-third of DEGs, most of which were CHH-type DMRs, were associated with DMRs. Functional analysis of the CHH DMR-DEGs indicated their involvement in many important biological processes, including cell communication and response to external stimulus. The transcription of many NBS-LRR family genes was affected by changes in DNA methylation, suggesting that DNA methylation plays essential roles in the response of rice to M. oryzae infection. More broadly, the DNA methylation analysis presented here sheds light on epigenomic involvement in plant defense against fungal pathogens. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.