Distinction Between Two Populations of Islet-1-Positive Cells in Hearts of Different Murine Strains
Distinction Between Two Populations of Islet-1-Positive Cells in Hearts of Different Murine Strains
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DOI：
10.1089/scd.2010.0374
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发表时间：
2011-06-01
影响因子：
4
通讯作者：
Vilquin, Jean-Thomas
中科院分区：
文献类型：
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作者：
Khattar, Patricia;Friedrich, Felix W.;Vilquin, Jean-Thomas
胰岛-1的表达确定了胚胎、胎儿和新生小鼠心脏中的祖细胞群体，这些祖细胞能够在体内和体外产生所有心脏细胞谱系。利用系统免疫组织化学，我们从胰岛-1阳性细胞潜在的治疗用途的角度研究了成年小鼠心脏中是否存在胰岛-1阳性细胞。在不同品系、年龄和条件的小鼠中，评估了胰岛1阳性细胞的存在、定位和性质。胰岛-1阳性细胞从出生第1天到成年后一直存在于小鼠心脏中。根据菌株的不同，这些细胞被组织成1或2种类型的集群，这些集群定位于限定区域，距离基部心脏长度的6%-35%。第一种类型的细胞簇存在于所有品系中，由形成心脏神经节的神经嵴来源细胞组成。从新生儿到成年，细胞数量保持稳定（几百个），并且在它们的长期持久性方面，菌株之间存在差异。第二类聚集型主要存在于129SvJ或Balb/C株中，而不存在于C57BL/6J、C3H、SJL株中。它由表达高度有序的肌动蛋白的细胞组成，与它们具有心肌细胞身份一致。这些细胞在4个月大的动物身上消失了。在扩张型心肌病小鼠模型中，胰岛1阳性细胞的数量和类型都没有随时间变化。我们的研究表明，胰岛1阳性细胞在成年小鼠心脏中数量相对较少，定位于受限且难以接近的区域，并且可以代表神经嵴和心肌细胞谱系。
Islet-1 expression identifies populations of progenitor cells in embryonic, fetal, and newborn murine hearts that are able to give rise to all cardiac cell lineages ex vivo and in vivo. Using systematic immunohistochemistry, we investigated whether islet-1-positive cells are present in adult mouse heart from the perspective of their potential therapeutic utility. The presence, localization, and nature of islet-1-positive cells were assessed in mice of different strains, ages, and conditions. Islet-1-positive cells were present in mouse heart from postnatal day 1 to young adulthood. Depending on the strain, these cells were organized in either 1 or 2 types of clusters localized to restricted areas, at a distance of 6%-35% of the heart length from the base. The first type of cluster was present in all strains and consisted of neural crest-derived cells that formed cardiac ganglia. The number of cells remained stable (a few hundred) from neonatal up to adult ages, and variations were noted between strains regarding their long-term persistency. The second type of cluster was essentially present in 129SvJ or Balb/C strains and absent from the other strains tested (C57BL/6J, C3H, SJL). It consisted of cells expressing highly ordered sarcomeric actin, consistent with their having cardiomyocyte identity. These cells disappeared in animals older than 4 months. Neither the number nor the type of islet-1-positive cells varied with time in a mouse model of dilated cardiomyopathy. Our studies demonstrate that islet-1-positive cells are relatively few in number in adult murine heart, being localized in restricted and rather inaccessible areas, and can represent both neural crest and cardiomyocyte lineages.