Multiple actin-related sequences in the Drosophila melanogaster genome
Multiple actin-related sequences in the Drosophila melanogaster genome
复制标题
果蝇基因组中多个肌动蛋白相关序列
DOI：
10.1016/0092-8674(80)90393-1
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发表时间：
1980
期刊：
影响因子：
64.5
通讯作者：
B. Mccarthy
中科院分区：
文献类型：
--
作者：
S. Tobin;E. Zulauf;F. Sánchez;E. Craig;B. Mccarthy
本文报道了一株7.2kb的D.黑腹果蝇染色体DNA片段（K1），其含有与黑腹果蝇互补的核苷酸序列。黑腹肌动蛋白mRNA。质粒K1使用网骨藻肌动蛋白cDNA质粒B1作为探针来鉴定。D.通过与固定化K1 DNA杂交选择的黑腹果蝇mRNA在体外翻译产生与D.黑腹肌动蛋白I、II和III在二维凝胶电泳中显示，并与DNA酶I琼脂糖结合。一个物理地图定位限制性内切酶切割位点的Ki DNA片段和转录的方向。通过与标记的B1 DNA和标记的含poly（A）的D的杂交，定位了编码区的位置。黑腹果蝇RNA。根据质粒K1的标记亚片段与限制性内切酶切割的D.通过对黑腹果蝇胚胎DNA的序列分析，我们得出结论，推测的编码区的核苷酸序列负责标记胚胎DNA的多个限制性片段的模式。DNA片段K1所来源的染色体位点已通过原位杂交定位于88F区的两条紧密连锁的带。与假定的肌动蛋白基因相对应的相关DNA序列也已在细胞学上作图。这些结果支持了D.黑腹果蝇是编码序列的密切相关家族的成员。
In this paper we describe the isolation and characterization of a 7.2 kb D. melanogaster chromosomal DNA fragment (Kl) which contains nucleotide sequences complementary to D. melanogaster actin mRNA. Plasmid Kl was identified using a Dictyostelium actin cDNA plasmid, Bl, as a probe. D. melanogaster mRNA selected by hybridization with immobilized Kl DNA was translated in vitro to yield products which co-migrate with the D. melanogaster actins I, II and Ill in two-dimensional gel electrophoresis and bind to DNAase I agarose. A physical map localizing restriction endonuclease cleavage sites in the Ki DNA fragment and the direction of transcription is presented. The position of the coding region has been localized by hybridization with labeled Bl DNA and with labeled poly (A)-containing D. melanogaster RNA. On the basis of hybridization of labeled subfragments of plasmid Kl to restriction endonuclease-cleaved D. melanogaster embryo DNA, we conclude that the nucleotide sequence of the presumptive coding region is responsible for labeling of a pattern of multiple restriction frag ments from embryo DNA. The chromosomal locus from which DNA fragment Kl is derived has been localized by in situ hybridization to two closely linked bands in the region 88F. Related DNA sequences corresponding to putative actin genes have also been mapped cytologically. These results support the hypothesis that the genes for actin in D. melanogaster are members of a closely related family of coding sequences.