Murine Endometrial and Decidual NK1.1+ Natural Killer Cells Display a B220+CD11c+ Cell Surface Phenotype
Murine Endometrial and Decidual NK1.1+ Natural Killer Cells Display a B220+CD11c+ Cell Surface Phenotype
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DOI：
10.1095/biolreprod.109.076448
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发表时间：
2009-08-01
影响因子：
3.6
通讯作者：
Riley, Joan K.
中科院分区：
文献类型：
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作者：
Mallidi, Thomas V.;Craig, Laura E.;Riley, Joan K.
子宫自然杀伤（uNK）细胞在妊娠期间在母胎界面处积累，并且被认为在小鼠和人类的妊娠期间具有重要作用。虽然人uNK细胞的细胞表面表型越来越明确，但关于妊娠前和妊娠期间鼠uNK细胞的细胞表面表达谱知之甚少。在此，我们证明了来源于处女小鼠的小鼠NK1.1（+）（KLRB1C）子宫内膜NK（eNK）细胞和来源于妊娠小鼠的NK1.1（+）蜕膜NK（dNK）细胞属于NK细胞的B220（+）（PTPRC）CD11c+（ITGAX）亚群。虽然NK1.1（+）eNK细胞上的B220表达较低，但在胚胎第10.5天，NK1.1（+）dNK细胞亚组上的B220表达增加。子宫内膜NK细胞和dNK细胞的两种活化标志物，即CD69和诱导型共刺激分子（ICOS）的表达模式也有所不同。当在子宫细胞和鼠白细胞介素15存在下体外培养时，eNK细胞获得B220（hi）ICOS（+）dNK细胞表面表型。因此，NK1.1（+）eNK细胞和dNK细胞生成的细胞表面特征证明它们属于最近描述的NK细胞的B220（+）CD11c（+）亚群，其是强效细胞因子产生者。
Uterine natural killer (uNK) cells accumulate at the maternal-fetal interface during gestation and are thought to have an important role during pregnancy in both mice and humans. While the cell surface phenotype of human uNK cells is increasingly well defined, less is known regarding the cell surface expression profile of murine uNK cells both before and during gestation. Herein, we demonstrate that murine NK1.1(+) (KLRB1C) endometrial NK (eNK) cells, derived from virgin mice, and NK1.1(+) decidual NK (dNK) cells, obtained from pregnant mice, belong to the B220(+) (PTPRC) CD11c+ (ITGAX) subset of NK cells. While B220 expression was low on NK1.1(+) eNK cells, it was increased on a subset of NK1.1(+) dNK cells at Embryonic Day 10.5. Endometrial NK and dNK cells also differed somewhat in their expression patterns of two activation markers, namely, CD69 and inducible costimulator (ICOS). The eNK cells acquired a B220(hi)ICOS(+) dNK cell surface phenotype when cultured in vitro in the presence of uterine cells and murine interleukin 15. Thus, the cell surface profiles generated for both NK1.1(+) eNK cells and dNK cells demonstrate that they belong to the recently described B220(+) CD11c(+) subset of NK cells, which are potent cytokine producers.