Unilateral cortical application of tumor necrosis factor α induces asymmetry in Fos- and interleukin-1β-immunoreactive cells within the corticothalamic projection
Unilateral cortical application of tumor necrosis factor α induces asymmetry in Fos- and interleukin-1β-immunoreactive cells within the corticothalamic projection
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DOI：
10.1016/j.brainres.2005.06.052
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发表时间：
2005-09-07
期刊：
影响因子：
2.9
通讯作者：
Kmeger, JM
中科院分区：
文献类型：
--
作者：
Churchill, L;Yasuda, K;Kmeger, JM
将肿瘤坏死因子α（TNF α）（150 ng）单侧显微注射到初级体感皮层上，会在大鼠非快速眼动睡眠期间诱导脑电图（EEG）慢波活动的状态依赖性不对称性[H. Yoshida，Z. Peterfi，F. Garcia-Garcia，R. Kirkpatrick，T. Yasuda，J.M. Krueger，局部应用 TNF α 引起的脑电图慢波活动的状态特异性不对称性，Brain Res。 1009（2004）129-136]。在当前的研究中，进行类似的 TNF α 注射以确定 Fos 和白细胞介素 1 β (IL1 β) 免疫反应性 (IR)。相对于接受热灭活 TNF α 的对侧，单侧显微注射 TNF α 增加了初级体感皮层中 Fos 和 IL1 β-IR 细胞的数量。这些不对称 TNF α 诱导的 Fos 和 IL1 β-IR 细胞数量的增加沿着皮质外表面（主要是第 II 层和第 III 层）在限制的头侧到尾侧区域中是明显的。在接受体感皮层主要皮质投射的皮层下区域，即丘脑网状核（网状丘脑）的体细胞区域，也观察到 Fos-IR 细胞数量的不对称增加。 IL1 beta-IR 细胞用胶质纤维酸性蛋白 (GFAP) 双标记，表明许多 IL1 beta-IR 细胞是星形胶质细胞。注射TNFα的同侧网状丘脑中IL1β-IR细胞的数量显着增加。目前的结果表明，Fos-和 IL1 beta-IR 可用于研究与 TNF α 介导的状态依赖性脑电图慢波活动增强有关的功能神经解剖学。 (c) 2005 Elsevier B.V. 保留所有权利。
A unilateral microinjection of tumor necrosis factor alpha (TNF alpha) (150 ng) onto the primary somatosensory cortex induces state-dependent asymmetries in electroencephalographic (EEG) slow wave activity during non-rapid eye movement sleep in rats [H. Yoshida, Z. Peterfi, F. Garcia-Garcia, R. Kirkpatrick, T. Yasuda, J.M. Krueger, State-specific asymmetries in EEG slow wave activity induced by local application of TNF alpha, Brain Res. 1009 (2004) 129-136]. In the current study, analogous TNF alpha injections were performed to determine Fos- and interleukin-1 beta (IL1 beta) immunoreactivity (IR). A unilateral microinjection of TNF alpha increased the number of Fos- and IL1 beta-IR cells in the primary somatosensory cortex relative to the contralateral side that received heat-inactivated TNF alpha These asymmetric TNF alpha-induced increases in the number of Fos- and IL1 beta-IR cells were evident along the outside surface of the cortex (mainly layers II and III) in a restricted rostral to caudal zone. Asymmetrical increases in the number of Fos-IR cells were also observed in the subcortical region that receives the main cortical projection from the somatosensory cortex, the somatic region of the reticular nucleus of the thalamus (reticular thalamus). The IL1 beta-IR cells double-labeled with glial fibrillary acidic protein (GFAP), suggesting that many of the IL1 beta-IR cells were astrocytes. The number of the IL1 beta-IR cells in the reticular thalamus increased significantly ipsilateral to the TNF alpha injection. Current results indicated that Fos- and IL1 beta-IR may be utilized to study the functional neuroanatomy involved in the TNF alpha-mediated state-dependent enhancement of EEG slow wave activity. (c) 2005 Elsevier B.V. All rights reserved.