Detection of apoptosis in colorectal carcinoma by light microscopy and in situ end labelling.
Detection of apoptosis in colorectal carcinoma by light microscopy and in situ end labelling.
复制标题
通过光学显微镜和原位末端标记检测结直肠癌中的细胞凋亡。
DOI：
--
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
R. Ward
中科院分区：
文献类型：
--
作者：
N. Hawkins;J. Lees;R. Ward
目的
原位末端标记反应（ISEL）最近被描述用于检测组织切片中的凋亡细胞。我们比较了常规光学显微镜下使用苏木精和伊红（H&E）在定量结直肠癌中凋亡细胞的效率和重复性。
研究设计
研究中使用了15例结直肠癌的石蜡包埋存档组织。使用末端脱氧核苷酸转移酶、生物素化脱氧尿苷三磷酸和链霉亲和素-碱性磷酸酶进行ISEL。
结果
通过两种测定法确定的凋亡指数显示出强的正相关性（Pearson系数0.82），其中ISEL测定法检测到的凋亡细胞的数量是用H&E染色观察到的凋亡细胞的数量的两倍。这两种检测都显示出显著的观察者间和观察者内变异，而ISEL检测也显示出相当大的检测间变异性，并且在检测凋亡细胞方面的成本效益低于H&E。
结论
在结肠直肠肿瘤细胞凋亡的光镜定量中，ISEL测定没有提供更大的可重复性，并且比常规H&E染色的成本效益低。
OBJECTIVE
An in situ end labelling reaction (ISEL) was recently described for the detection of apoptotic cells in histologic sections. We compared the efficiency and reproducibility of this assay with those of routine light microscopy using hematoxylin and eosin (H&E) in the quantitation of apoptotic cells in colorectal cancer.
STUDY DESIGN
Paraffin-embedded archival tissues from 15 colorectal carcinomas were used in the study. ISEL was performed using terminal deoxynucleotidyl transferase, biotinylated deoxyuridine triphosphate and streptavidin-alkaline phosphatase.
RESULTS
Apoptotic indices determined by the two assays showed a strong positive correlation (Pearson coefficient 0.82), with the ISEL assay detecting twice the number of apoptotic cells as that seen with H&E staining. Both assays showed significant interobserver and intraobserver variation, while the ISEL assay also showed considerable interassay variability and was less cost-effective than H&E in the detection of apoptotic cells.
CONCLUSION
In the light microscopic quantitation of apoptosis in colorectal tumors, the ISEL assay offered no greater reproducibility, and was less cost-effective, than routine H&E staining.