Impact of short-term oral dose of cinnamtannin A2, an (-)-epicatechin tetramer, on spatial memory and adult hippocampal neurogenesis in mouse
Impact of short-term oral dose of cinnamtannin A2, an (-)-epicatechin tetramer, on spatial memory and adult hippocampal neurogenesis in mouse
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短期口服肉桂单宁 A2（一种 (-)-表儿茶素四聚体）对小鼠空间记忆和成年海马神经发生的影响
DOI：
10.1016/j.bbrc.2021.11.021
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发表时间：
2021
影响因子：
3.1
通讯作者：
Osakabe Naomi
中科院分区：
文献类型：
--
作者：
Fujii Yasuyuki;Sakata Jun;Sato Fumitaka;Onishi Kurumi;Yamato Yuki;Sakata Kazuki;Taira Shu;Sato Hiroki;Osakabe Naomi
最近的流行病学和干预研究表明，富含多酚的植物性食物消费减少了认知能力下降的风险。然而，调查结果是初步的，绝不是确定的。在本研究中，我们研究了短期口服肉桂单宁A2（A2）（一种（-）-表儿茶素四聚体）对小鼠成年海马神经发生和认知功能的影响。小鼠接受媒介物（20%甘油）或100 μg/kg A2的补充，持续10天。然后，我们进行了开放领域的测试，对象的位置测试，和新的对象测试。在旷场试验中，与溶剂处理组相比，A2处理组倾向于在竞技场中心花费更多时间。与媒介物处理组相比，A2处理组花费显著更多的时间探索放置在不同位置的物体。在物体偏好指数或新物体测试中，两组之间没有显著差异。此外，A2管理显着增加海马溴脱氧尿苷标记的细胞在齿状回的数量，但不是在CA 1或CA 3区。这些结果表明，短期给予A2可能通过增强成年小鼠齿状回的神经发生来影响空间记忆。
Recent epidemiological and intervention studies have suggested that polyphenol-rich plant food consumption reduced the risk of cognitive decline. However, the findings were tentative and by no means definitive. In the present study, we examined the impact of short-term oral administration of cinnamtannin A2 (A2), an (−)-epicatechin tetramer, on adult hippocampal neurogenesis and cognitive function in mice. Mice received supplementation with vehicle (20% glycerol) or 100 μg/kg A2 for 10 days. Then, we conducted the open field test, the object location test, and the novel object test. In the open field test, the A2-treated group tended to spend more time in the center of the arena, compared to the vehicle-treated group. The A2-treated group spent significantly more time exploring objects placed in different locations, compared to the vehicle-treated group. There were no significant differences between groups in the object preference index or in the novel object test. In addition, A2 administration significantly increased the number of hippocampal bromodeoxyuridine-labeled cells in the dentate gyrus, but not in the CA1 or CA3 regions. These results suggested that short-term administration of A2 may impact spatial memory by enhancing neurogenesis in the dentate gyrus of adult mice.