Origin and development of adventitious shoot meristems initiated on plant chimeras.
Origin and development of adventitious shoot meristems initiated on plant chimeras.
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DOI：
10.1006/dbio.1993.1023
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发表时间：
1993
影响因子：
2.7
通讯作者：
H. Tian;M. Marcotrigiano
中科院分区：
文献类型：
--
作者：
H. Tian;M. Marcotrigiano
大多数关于茎尖分生组织发生和发育的研究都是在胚胎发生过程中发育的分生组织上进行的。我们描述了一系列六个种间平周烟草嵌合体中源自叶腋附近细胞的不定芽的原位形成。通过将植物去顶并去除所有腋芽来产生芽。413个再生芽中有84个为嵌合体。许多芽是复杂的分生组织嵌合体，其中几个腋芽具有排列为平周嵌合体的分生组织。不定芽起源于源植物的分生组织中仅第二和第三细胞层的衍生物，因为最外面的分生组织层的衍生物随着腋芽的去除而被去除。随着时间的推移，几乎所有的不定芽尖，最初嵌合成为nonchimeral或稳定的平周嵌合体。统计分析表明，遗传上不同的组织在不定分生组织的细胞层中的排列受到细胞类型之间竞争的影响。我们产生这些芽的方法可用于创建类似于辐射诱导的部门的小的遗传上不同的部门以及一系列完整的临床周围嵌合体，这两者都有可能用于确定组织间的相互作用。嵌合芽的扇形长度和位置的分析表明，不定芽的第一到三片叶不出现作为一个分生组织适当的芽顶端原始的衍生物。
Most studies concerning the initiation and development of the shoot apical meristem have been performed on meristems that developed during embryogenesis. We characterized the in situ formation of adventitious shoots originating from cells located near leaf axils in a series of six interspecific periclinal tobacco chimeras. Shoots were generated by decapitating the plants and removing all of the axillary buds. Eighty-four of the 413 shoots regenerated were chimeral. Many of the shoots were complex mericlinal chimeras, with several of their axillary buds possessing meristems arranged as periclinal chimeras. The adventitious shoots originated from derivatives of only the second and third cell layers in the meristem of the source plant since derivatives of the outermost meristem layer were removed with the removal of the axillary buds. With time, nearly all adventitious shoot apices that were initially chimeral became nonchimeral or stabilized as periclinal chimeras. A statistical analysis indicated that the arrangement of genetically distinct tissues in the cell layers of adventitious meristems was influenced by competition between cell types. Our method for generating these shoots can be used to create small genetically distinct sectors analogous to radiation-induced sectors as well as a complete series of periclinical chimeras, both of which have potential for use in determining tissue-to-tissue interactions. An analysis of sector length and position on chimeral shoots indicates that the first one to three leaves of adventitious shoots do not arise as derivatives of the shoot apical initials of a meristem proper.