Arbuscular mycorrhizal fungi combined with exogenous calcium improves the growth of peanut (Arachis hypogaea L.) seedlings under continuous cropping
Arbuscular mycorrhizal fungi combined with exogenous calcium improves the growth of peanut (Arachis hypogaea L.) seedlings under continuous cropping
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丛枝菌根真菌联合外源钙促进花生连作幼苗生长
DOI：
10.1016/s2095-3119(19)62611-0
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发表时间：
2019-02-01
影响因子：
4.8
通讯作者：
Wan Shu-bo
中科院分区：
文献类型：
--
作者：
Cui Li;Guo Feng;Wan Shu-bo
连作对花生的生长发育和产量有一定的负面影响。丛枝菌根真菌（AMF）和钙离子（Ca 2+）已被用于提高其他植物的抗逆性，但它们在连作花生幼苗生长中的作用知之甚少。本研究探讨了丛枝菌根真菌Glomus mosseae与外源Ca ~（2+）复合对连作花生幼苗生理响应的可能作用。G. mosseae与外源Ca ~（2+）配施能提高植株生物量、Ca ~（2+）水平和叶绿素总量。外源Ca ~（2+）处理下，高光照条件下丛枝菌根（AM）植物叶片的F-v/F-m均高于对照。AM植物叶片中过氧化物酶、超氧化物歧化酶和过氧化氢酶活性也达到最大值，相应地，丙二醛含量最低。另外，Ca 2+处理的AM植物根系活力、总酚和类黄酮含量显著高于G. mosseae接种或单独Ca ~（2+）处理。外源Ca（2+）处理显著提高了AM植物根系AhCaM、AhCDPK、AhRAM 1和AhRAM 2的转录水平。这表明外源Ca ~（2+）可能参与了G. mosseae在花生植株上的定殖，而AM共生可能激活了Ca ~（2+）信号转导途径。AMF与Ca ~（2+）配合使用有利于连作条件下植物的生长发育，是一种很有前途的科普连作胁迫的方法。
The growth and yield of peanut are negatively affected by continuous cropping. Arbuscular mycorrhizal fungi (AMF) and calcium ions (Ca2+) have been used to improve stress resistance in other plants, but little is known about their roles in peanut seedling growth under continuous cropping. This study investigated the possible roles of the AMF Glomus mosseae combined with exogenous Ca2+ in improving the physiological responses of peanut seedlings under continuous cropping. G. mosseae combined with exogenous Ca2+ can enhance plant biomass, Ca2+ level, and total chlorophyll content. Under exogenous Ca2+ application, the F-v/F-m in arbuscular mycorrhizal (AM) plant leaves was higher than that in the control plants when they were exposed to high irradiance levels. The peroxidase, superoxide dismutase, and catalase activities in AM plant leaves also reached their maximums, and accordingly, the malondialdehyde content was the lowest compared to other treatments. Additionally, root activity, and content of total phenolics and flavonoids were significantly increased in AM plant roots treated by Ca2+ compared to either G. mosseae inoculation or Ca2+ treatment alone. Transcription levels of AhCaM, AhCDPK, AhRAM1, and AhRAM2 were significantly improved in AM plant roots under exogenous Ca(2+ )treatment. This implied that exogenous Ca2+ might be involved in the regulation of G. mosseae colonization of peanut plants, and in turn, AM symbiosis might activate the Ca2+ signal transduction pathway. The combination of AMF and Ca2+ benefitted plant growth and development under continuous cropping, suggesting that it is a promising method to cope with the stress caused by continuous cropping.