Microbial Lipid Production from Corn Stover by the Oleaginous Yeast Rhodosporidium toruloides Using the PreSSLP Process
Microbial Lipid Production from Corn Stover by the Oleaginous Yeast Rhodosporidium toruloides Using the PreSSLP Process
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DOI：
10.3390/en12061053
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发表时间：
2019-03-19
期刊：
影响因子：
3.2
通讯作者：
Zhao, Zongbao K.
中科院分区：
文献类型：
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作者：
Dai, Xiaozan;Shen, Hongwei;Zhao, Zongbao K.
干酸预处理和生物解毒（DryPB）被认为是处理木质纤维素材料以改善下游生物转化的先进技术。在这项研究中，通过产油酵母圆红冬孢酵母，使用预水解随后同步糖化和脂质生产 (PreSSLP) 的新工艺研究了 DryPB 玉米秸秆的脂质生产。结果发现，与没有预水解步骤的相比，在50℃预水解，然后在30℃生产脂质，脂质产率提高了17.0%以上。当固含量为 12.5% 时，最高脂质产量为 0.080 g/g DryPB 玉米秸秆。脂质产品的脂肪酸分布与用于生物柴油生产的传统植物油的脂肪酸分布相似。我们的结果表明，DryPB 工艺和 PreSSLP 工艺的整合可以作为木质纤维素材料微生物脂质生产的改进技术进行探索。
Dry acid pretreatment and biodetoxification (DryPB) has been considered as an advanced technology to treat lignocellulosic materials for improved downstream bioconversion. In this study, the lipid production from DryPB corn stover was investigated by the oleaginous yeast Rhodosporidium toruloides using a new process designated prehydrolysis followed by simultaneous saccharification and lipid production (PreSSLP). The results found that prehydrolysis at 50 degrees C and then lipid production at 30 degrees C improved lipid yield by more than 17.0% compared with those without a prehydrolysis step. The highest lipid yield of 0.080 g/g DryPB corn stover was achieved at a solid loading of 12.5%. The fatty acid distribution of lipid products was similar to those of conventional vegetable oils that are used for biodiesel production. Our results suggested that the integration of DryPB process and PreSSLP process can be explored as an improved technology for microbial lipid production from lignocellulosic materials.