CEREBELLAR GRANULE CELL GENESIS IN THE HYDROCORTISONE-TREATED RAT
CEREBELLAR GRANULE CELL GENESIS IN THE HYDROCORTISONE-TREATED RAT
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DOI：
10.1159/000112380
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发表时间：
1980-01-01
影响因子：
2.9
通讯作者：
LAUDER, JM
中科院分区：
文献类型：
--
作者：
BOHN, MC;LAUDER, JM
通过定量光学显微镜方法研究了出生后 7-18 天接受氢化可的松治疗的大鼠小脑皮质的发育。这种处理导致外部颗粒层 (EGL) 中的细胞数量减少，并且 EGL 提早消失。在这些新生动物的成年小脑中，第 VIII 小叶中的颗粒细胞总数减少了 41%，分子层微神经元总数减少了 28%。小脑微神经元（起源时间）的放射自显影测定表明，内部颗粒层和分子层细胞形成的峰值出现在比对照组更早的年龄。在颗粒细胞群中，这一观察结果是治疗期间细胞增殖减少和 EGL 中颗粒细胞前体细胞分裂过早停止的结果。本研究中观察到的晚期氢化可的松治疗对小脑的形态学影响与早期氢化可的松治疗第 1-4 天的影响以及先前研究中甲状腺激素的影响进行了比较。新生儿期甲状腺激素和糖皮质激素的内源水平可能会影响出生后大鼠小脑的神经发生率。
The development of the cerebellar cortex was studied by quantitative light microscopic methods in rats treated with hydrocortisone in postnatal days 7-18. This treatment resulted in a decreased number of cells in the external granular layer (EGL) and an early disappearance of the EGL. In the adult cerebellum of these neonatally treated animals, the total number of granule cells in lobule VIII was decreased by 41% and that of molecular layer microneurons by 28%. Autoradiographic determinations of the (time of origin) of cerebellar microneurons showed that the peaks of cell formation in the internal granular and molecular layers occurred at earlier ages than in controls. In the granule cell population, this observation was the result of both decreased cell proliferation during the treatment and premature cessation of division in granule cell precursors in the EGL. The morphological effects of late hydrocortisone treatment on the cerebellum observed in this study are compared with those of early hydrocortisone treatment on days 1-4 and those of thyroid hormone from previous studies. Endogenous levels of both thyroid and glucocorticoid hormones during the neonatal period may influence the rate of neurogenesis in the postnatal rat cerebellum.