Prevalence, antibiotic resistance and molecular characterisation of Staphylococcus aureus in pigs at agricultural fairs in the USA
Prevalence, antibiotic resistance and molecular characterisation of Staphylococcus aureus in pigs at agricultural fairs in the USA
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DOI：
10.1136/vr.100570
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发表时间：
2012-05-01
影响因子：
2.2
通讯作者：
Smith, T. C.
中科院分区：
文献类型：
--
作者：
Dressler, A. E.;Scheibel, R. P.;Smith, T. C.
集市和宠物动物园与人畜共患病的爆发有关。此前，有记录表明商品猪中存在耐甲氧西林金黄色葡萄球菌（MRSA）;因此，假设农业博览会上展出的猪也可能出现耐抗生素金黄色葡萄球菌。为了验证这一假设，在2008年至2009年的两个州集市上，对157头猪进行了拭子采样。对两个鼻孔取样，培养物在增菌肉汤中生长，然后铺在选择性MRSA平板和血平板上。金黄色葡萄球菌确认使用表型和分子方法，并进行了分析，使用SPA分型，基因特异性聚合酶链反应和抗生素敏感性试验。从美国猪展上展出的猪采集的样本中证实存在金黄色葡萄球菌。157个样本中有25个（15.9%）对金黄色葡萄球菌呈阳性。两个分离株（8%）对甲氧西林耐药; 23/25（92%）、14/25（56%）和15/25（60%）分别对四环素、红霉素和克林霉素耐药。spa分型显示多个spa型分离株为t034（9/25，36%）和t337（7/25，28%），以及t002，t209，t526，t1236，t1334，t1683，t3075，t5784和t5883的单株。这些结果证实了在美国博览会上展出的猪中存在耐药性金黄色葡萄球菌，表明猪是这种环境中金黄色葡萄球菌的潜在储存库。
Fairs and petting zoos have been associated with outbreaks of zoonotic disease. Previously, the presence of meticillin-resistant Staphylococcus aureus (MRSA) was documented in commercial pigs; therefore, it was hypothesised that antibiotic-resistant S aureus may also occur in pigs exhibited at agricultural fairs. To test this hypothesis, 157 pigs were swabbed at two state fairs in 2008 to 2009. Both nares were sampled and cultures were grown in enrichment broth, then plated onto selective MRSA plates and blood plates. S aureus was confirmed using phenotypic and molecular methods, and was analysed using spa typing, gene-specific polymerase chain reaction and antibiotic susceptibility testing. The presence of S aureus was confirmed in samples collected from pigs exhibited at USA pig shows. Twenty-five of 157 (15.9 per cent) samples were positive for S aureus. Two isolates (8 per cent) were resistant to meticillin; 23/25 (92 per cent), 14/25 (56 per cent) and 15/25 (60 per cent) were resistant to tetracycline, erythromycin and clindamycin, respectively. spa typing revealed multiple isolates of spa type t034 (9/25, 36 per cent) and t337 (7/25, 28 per cent) and singletons of t002, t209, t526, t1236, t1334, t1683, t3075, t5784 and t5883. These results verify the presence of antibiotic-resistant S aureus in pigs exhibited at USA fairs, suggesting that pigs are a potential reservoir for S aureus within this environment.