Genetics of host-pathogen relationships between Venturia inaequalis races 6 and 7 and Malus species
Genetics of host-pathogen relationships between Venturia inaequalis races 6 and 7 and Malus species
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DOI：
10.1094/phyto.2000.90.3.236
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发表时间：
2000-03-01
期刊：
影响因子：
3.2
通讯作者：
Parisi, L
中科院分区：
文献类型：
--
作者：
Bénaouf, G;Parisi, L
对黄斑病的抗性源自苹果无性系821，是苹果育种中应用最广泛的抗性形式。一个名为Vf的显性基因从该无性系渗入到最近的栽培品种中，尽管决定花霉821抗性的遗传因素显然比单一基因更为复杂。新品种的出现克服了具有Vf基因的品种、亲本无性系或两者的抗性，使得对宿主-病原体相互作用进行遗传分析成为可能。“金冠”与“金冠”与“Idared”杂交后代的抗性分离为5个不均匀文图里亚菌株，即1种（菌株104、1093和301）、6种（菌株302）和7种（菌株1066），证明了在“金冠”与“花冠”821中存在第二显性基因。这种独立于Vf的基因被命名为Vfh，因为它似乎会引起过敏反应。对M， floribunda 821有毒力的菌株1066获得的结果，允许鉴定另一个优势基因Vg，负责“金冠”对该菌株的抗性。“Florina”也携带Vg，选择它是因为它具有抗Vf的能力。在不同基因型的叶片上，对不平等弧菌1066和301杂交后代的致病性进行了离体鉴定，发现两个独立的无毒基因分别参与了对Vg和VS基因的致病性。这些无毒基因分别被命名为Avr Vg和Avr Vf。检测到的宿主-病原体相互作用与基因对基因的关系一致。
Resistance to scab originating from Malus floribunda clone 821 is the most widely form of resistance used in apple breeding programs. A dominant gene, named Vf, was introgressed from this clone into recent cultivars, although the genetic determinants of the resistance of M. floribunda 821 are apparently more complex than a single gene. The appearance of new races overcoming the resistance of cultivars with the Vf gene, the parental clone, or both made it possible to undertake a genetic analysis of host-pathogen interactions. The segregation of resistance in progenies of crosses from 'Golden Delicious' x M. floribunda 821 and 'Golden Delicious' x 'Idared' into five strains of Venturia inaequalis-races 1 (strains 104, 1093, and 301), 6 (strain 302), and 7 (strain 1066)-demonstrated the existence of a second dominant gene in M. floribunda 821. This gene, independent of Vf, was named Vfh because it seemed to induce a hypersensitive reaction. The results obtained with strain 1066, virulent to M, floribunda 821, allowed identification of another dominant gene, Vg, responsible for the resistance of 'Golden Delicious' to this strain. Vg is also carried by 'Florina', which was selected for its Vf resistance. The pathogenicity of a progeny originating from a cross between V. inaequalis strains 1066 and 301, characterized in vitro on leaf disks of differential genotypes, revealed two independent avirulence genes involved in the pathogenicity toward the Vg and VS genes, respectively. These avirulence genes were named Avr Vg and Avr Vf. The host-pathogen interactions detected are consistent with a gene-for-gene relationship.