Comparative sequence and expression analysis of tapetum specific male sterility related genes in Medicago truncatula.
Comparative sequence and expression analysis of tapetum specific male sterility related genes in Medicago truncatula.
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DOI：
10.4238/gmr.15028323
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发表时间：
2016-07
期刊：
影响因子：
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通讯作者：
Shao Lh;Zheng Xw;Yi Dx;C. Li
中科院分区：
文献类型：
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作者：
Shao Lh;Zheng Xw;Yi Dx;C. Li
杂种优势，或通过近交改良，是提高作物产量最有前途的途径之一。促进杂种优势的雄性不育在杂交作物生产中得到了广泛的应用。元宝紫花苜蓿是一种模式豆科植物，与重要的豆科牧草紫花苜蓿亲缘关系密切。尽管元宝菊和紫花苜蓿MS的分子机制尚不清楚，但在拟南芥中已经进行了一些关于MS的研究。先前的研究表明，多发性硬化症与绒毛细胞层的破坏有关。绒毡层发育过程的中断可能导致花粉败育。为了找出对紫花苜蓿育种有用的基因，我们利用BLAST和与拟南芥基因的同源性，鉴定了元宝草的MS相关基因。在本研究中，我们鉴定了63个绒毡层特异雄性不育相关基因。Tsms基因的长度在225~3747bp之间。我们鉴定了15个保守结构域和8个与TSMS相关基因的顺式元件。对这些基因之间的系统发育关系进行了分析，将它们分为三个组，MtTsms A、MtTsms B和MtTsms C。表达分析表明，这些基因可能参与了发育过程和对非生物胁迫的响应。
Heterosis, or enhancement through outbreeding, is one of the most promising approaches for increasing crop yield. Male sterility (MS), which promotes heterosis, has been widely applied in hybrid crop production. Medicago truncatula is a model legume species and is closely related to M. sativa, an important legume forage plant. Although the molecular mechanisms of MS in M. truncatula and M. sativa remain unclear, several studies of MS have been conducted in Arabidopsis thaliana. Previous research has shown that MS is associated with the destruction of tapetal cell layers. Disruption of tapetum developmental processes may result in pollen abortion. In an effort to identify genes useful for breeding in M. sativa, we identified MS related genes in M. truncatula using BLAST and homology to A. thaliana genes. In this study, we identified 63 tapetum specific male sterility (TSMS) related genes. The length of TSMS genes varied from 225 to 3747 bp. We identified 15 conserved domains and 8 cis-elements associated with TSMS related genes. Analysis of the phylogenetic relationships among these genes allowed them to be classified into three groups, MtTsms A, MtTsms B, and MtTsms C. Expression analyses revealed that these genes may be involved in developmental processes and response to abiotic stress.