Identification of astrovirus serotypes from children treated at the Hospitals for Sick Children, London 1981–93
Identification of astrovirus serotypes from children treated at the Hospitals for Sick Children, London 1981–93
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1981-93 年伦敦病童医院治疗儿童的星状病毒血清型鉴定
DOI：
10.1017/s0950268800051578
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发表时间：
1994
影响因子：
4.2
通讯作者：
D. Cubitt
中科院分区：
文献类型：
--
作者：
J. Noel;D. Cubitt
应用星状病毒1型的酶免疫测定（EIA）和免疫电镜（IBM）对1981-93年从腹泻儿童中获得的162种星状病毒进行分型。发现EIA对1型星状病毒具有特异性。星状病毒2-4型通过IEM分型。星状病毒1型是流行的血清型107/125（86%），其次是3型（8%）、4型（6%）和2型（1%）。6个含有星状病毒的样品不能通过EIA分型或检测，因为它们被粪抗体包被;另外11个未被鉴定。储存≥ 2年后，15/162份（9%）样本中不再检出病毒颗粒。将含有星状病毒1-4型的选定样品在CaCO 2细胞中传代，并通过一种或两种测定法确认其身份。其中一个样品在-20 °C的水悬浮液中保存了10年。两名患有严重联合免疫缺陷病（SCID）的患者分别在32天和102天内分泌星状病毒1型。一个孩子同时排出轮状病毒，另一个孩子排出腺病毒。
SUMMARY An enzyme immunoassay (EIA) for astrovirus type 1 together with immune electronmicroscopy (IBM) was used to type a collection of 162 astroviruses obtained from 1981–93 from children with diarrhoea. The EIA was found to be specific for astrovirus type 1. Astrovirus types 2–4 were typed by IEM. Astrovirus type 1 was the prevalent serotype 107/125 (86%), followed by type 3 (8%), type 4 (6%) and type 2 (1 %). Six samples containing astrovirus could not be typed or detected by EIA because they were coated with coproantibodies; 11 others were not identified. Virus particles could no longer be detected in 15/162 (9%) samples following storage for ≥ 2 years. Selected samples containing astrovirus types 1–4 were passaged in CaCO2 cells and their identity confirmed by one or both assays. One sample was shown to have remained viable for 10 years when stored as an aqueous suspension at −20 °C. Two patients with severe combined immune deficiency disease (SCID) were shown to be excreting astrovirus type 1 for 32 and 102 days respectively. One child was simultaneously shedding rotavirus and the other child was excreting adenovirus.