CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.
CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.
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拟南芥胚胎中表达的 CLE19 调节子叶建立和胚乳发育
DOI：
10.1093/jxb/erv293
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发表时间：
2015-08
影响因子：
6.9
通讯作者：
Liu CM
中科院分区：
文献类型：
--
作者：
Xu TT;Ren SC;Song XF;Liu CM
CLE 19作为一个在胚中表达的CLV 3/ESR基因，调控胚中子叶的建立和胚乳中的核增殖和细胞化。胚和胚乳发育是种子形成中两个协调良好的发育过程;然而，涉及胚和胚乳相互作用的信号仍然知之甚少。之前已经显示CLAVATA 3/ESR相关19（CLE 19）肽能够以CLV 2依赖性方式触发根分生组织消耗。本研究利用拮抗肽技术探讨CLE 19在拟南芥种子发育中的作用。CLE 19在胚的子叶原基、下胚轴和根冠的表皮层中表达。携带在CLE 19调控元件控制下表达的拮抗性CLE 19 G6 T构建体的转基因植物表现出显性种子败育表型，在胚中具有缺陷的子叶建立以及胚乳中延迟的核增殖和细胞化。在胚乳特异性ALE 1启动子控制下，CLE 19 G6 T在拟南芥中的异位表达导致胚中子叶建立的类似缺陷，但对胚乳发育没有明显影响。因此，我们认为CLE 19可能作为一个移动的肽协调胚和胚乳发育。
CLE19, as an embryo-expressed CLV3/ESR gene, regulates cotyledon establishment in embryos and nuclear proliferation and cellularization in the endosperm. Embryo and endosperm development are two well co-ordinated developmental processes in seed formation; however, signals involved in embryo and endosperm interactions remain poorly understood. It has been shown before that CLAVATA3/ESR-RELATED 19 (CLE19) peptide is able to trigger root meristem consumption in a CLV2-dependent manner. In this study, the role of CLE19 in Arabidopsis seed development was explored using antagonistic peptide technology. CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo. Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms. Ectopic expression of CLE19 G6T in Arabidopsis under the control of an endosperm-specific ALE1 promoter led to a similar defect in cotyledon establishment in embryos but without an evident effect on endosperm development. We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.