Hha, YbaJ, and OmpA regulate Escherichia coli K12 biofilm formation and conjugation plasmids abolish motility
Hha, YbaJ, and OmpA regulate Escherichia coli K12 biofilm formation and conjugation plasmids abolish motility
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DOI：
10.1002/bit.20681
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发表时间：
2006-01-05
影响因子：
3.8
通讯作者：
Wood, TK
中科院分区：
文献类型：
--
作者：
Barrios, AFG;Zuo, RJ;Wood, TK
大肠杆菌Hha是致病性溶血素操纵子的环境响应调节因子，Hha和相邻的YbaJ在大肠杆菌中均被诱导30倍。coli生物膜（Appl.Microbiol. Biotechnol. 64：515，2004）。在此显示，Hha和YbaJ调节生物膜形成，因为hha/ybaJ缺失减少了微量滴定板（基本培养基中81%，复合培养基中50%）和流动池（基本培养基中表面覆盖率少1，000倍）中的生物膜质量。去阻遏接合质粒R1 drd 19的加入，显著增加了生物膜的形成，完全消除了野生型大肠杆菌的运动性。coliK 12，促进细胞聚集27.18 +/- 0.05倍，并产生更平坦的生物膜。hha/ybaJ或ybaJ的缺失恢复了运动性（这种运动性表型可以通过提供反式的hha（+）/ybaJ（+）或ybaJ（+）来补充），并减少了细胞聚集至缺乏缀合质粒的野生型菌株。发现由于缺失hha/ybaJ导致的运动性的这种增加是由于fliA转录的8倍诱导。此外，缺失ompA在LB培养基和含葡萄糖的LB培养基中均使生物膜质量减少80%。此外，Hha/YbaJ促进缀合，因为在hha/ybaJ突变体中缀合少五倍。似乎接合质粒通过促进细胞聚集来促进生物膜形成，并且当接合质粒（R1 drd 19）存在时，Hha和YbaJ通过增加接合和通过降低运动性来增加生物膜形成（YbaJ在运动性的这种调节中起最重要的作用）。当hha/ybaJ缺失时，存在更少的缀合、更少的聚集、更多的运动性和更少的生物膜。(c)2005 Wiley Periodicals，Inc.
Escherichia coli Hha is an environmental-response regulator of the pathogenic hemolysin operon, and Hha and the contiguous YbaJ are both induced 30-fold in E. coli biofilms (Appl. Microbiol. Biotechnol. 64:515,2004). Here it is shown that Hha and YbaJ regulate biofilm formation since the hha/ybaJ deletion reduces biofilm mass in microtitre plates (81% in minimal medium, 50% in complex medium) and in flow cells (1,000-fold less surface coverage in minimal medium). The addition of the derepressed conjugative plasmid R1drd19, which increases significantly biofilm formation, eliminated motility completely in wild-type E. coli K12, promoted cell aggregation 27.18 +/- 0.05-fold, and produced a flatter biofilm. Deletion of hha/ybaJ or ybaJ restored motility (this motility phenotype may be complemented by providing hha(+)/ybaJ(+) or ybaJ(+) in trans) and reduced cell aggregation to that of the wild-type strain that lacks the conjugation plasmid. This increase in motility due to deleting hha/ybaJ was found to be due to 8-fold induction of fliA transcription. In addition, deletion of ompA reduced biofilm mass by 80% in both LB medium and LB medium with glucose. Also, Hha/YbaJ promotes conjugation since there was five-fold less conjugation in the hha/ybaJ mutant. It appears that conjugation plasmids promote biofilm formation by promoting cell aggregation, and that Hha and YbaJ increase biofilm formation by increasing conjugation and by decreasing motility when a conjugative plasmid (R1drd19) is present (YbaJ plays the most important role in this regulation of motility). When hha/ybaJ are deleted, there is less conjugation, less aggregation, more motility, and less biofilm. (c) 2005 Wiley Periodicals, Inc.