Synthesis and turnover of cell body-agglutinin as a pool of flagellar surface-agglutinin inChlamydomonas reinhardii gamete
Synthesis and turnover of cell body-agglutinin as a pool of flagellar surface-agglutinin inChlamydomonas reinhardii gamete
复制标题
莱茵衣藻配子中鞭毛表面凝集素库的细胞体凝集素的合成和周转
作者：
Tatsuaki Saito;Y. Tsubo;Y. Matsuda
在法国压力机中破碎莱茵衣藻细胞，并检测其可溶性部分的凝集活性。Mt+和mt-配子的去鞭毛细胞体产生了能够同凝集相反交配型配子的可溶性组分。当相反交配型的野生型配子混合时，在鞭毛凝集和随后的细胞融合过程中，细胞体凝集素被用完。当融合子mt+和mt-配子凝集而不连续融合时，细胞体凝集素的数量急剧减少，然后增加并达到预混合水平：放线菌素的恢复被放线菌酮阻断。细胞经EDTA或胰酶处理后，胞体凝集素和鞭毛表面凝集素完全消失，运动能力无明显变化。EDTA提取液中凝集素的含量与提取前细胞体中的凝集素含量相同，是分离鞭毛EDTA提取液中凝集素含量的100倍左右。通过添加胰酶抑制剂，经胰酶处理的配子重新合成细胞体凝集素。这一过程在交配型配子和衣霉素+配子中都对放线菌亚胺敏感。
Chlamydomonas reinhardii cells were broken in a French press and the soluble fraction was tested for agglutination activity. Deflagellated cell bodies ofmt+ andmt- gametes yielded soluble fractions that were able to isoagglutinate gametes of the opposite mating type. When the wild-type gametes of opposite mating types were mixed, the cell body-agglutinins were used up during flagellar agglutination and subsequent cell fusion. When thefus mt+ andmt- gametes agglutinated without successive fusion, the amount of cell body-agglutinins sharply decreased, then increased and reached the premixing level: the recovery was blocked by cycloheximide. When cells were treated with EDTA or trypsin, the cell body-agglutinins as well as flagellar surface-agglutinins were completely lost without apparent loss of motility. The EDTA extract contained the same amount of agglutinins as observed in the cell bodies before extraction, and this amount was about 100 times higher than that in the EDTA extract of isolated flagella. By the addition of trypsin inhibitor, the trypsinized gametes resynthesized the cell body-agglutinins. The process was sensitive to cycloheximide in both mating type gametes and to tunicamycin inmt+ gametes.
来自衣藻鞭毛膜的性凝集素。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Adair,WS;Monk,BC;Cohen,R;Hwang,C;Goodenough,UW
通讯作者：
Goodenough,UW