High terminal deoxynucleotidyl transferase activity in acute myelogenous leukemia.
High terminal deoxynucleotidyl transferase activity in acute myelogenous leukemia.
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发表时间：
1976-10
期刊：
影响因子：
11.2
通讯作者：
B. I. S. Srivastava;Sohaib A. Khan;Edward S. Henderson
中科院分区：
文献类型：
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作者：
B. I. S. Srivastava;Sohaib A. Khan;Edward S. Henderson
尽管所有13例急性淋巴细胞白血病患者的白细胞末端脱氧核苷酸转移酶均升高，（末端转移酶）活性（20至100单位/毫克细胞DNA，其中1单位等于1小时内聚合的1纳摩尔核苷酸），而来自21名急性髓细胞性白血病患者的那些具有低末端转移酶活性（0.2至2单位/mg细胞DNA），来自2名急性髓细胞白血病患者的骨髓和外周血白细胞，根据白血病细胞的临床特征和形态学、细胞化学和细胞遗传学诊断，具有与白血病淋巴母细胞中发现的相同的末端转移酶活性（39至52单位/mg细胞DNA）。这些结果带来的问题的特异性高终端转移酶活性以外的胸腺作为白血病淋巴母细胞的标记，其次，急性淋巴母细胞白血病细胞的衍生在所有情况下，从胸腺细胞。也许多能干细胞中的恶性转化以及末端转移酶基因组的去阻遏可以解释在某些白血病细胞中观察到的高末端转移酶活性。
Although leukocytes from all 13 acute lymphoblastic leukemia patients examined had high terminal deoxynucleotidyl transferase (terminal transferase) activity (20 to 100 units/mg of cellular DNA, where 1 unit equals 1 nmole of nucleotide polymerized in 1 hr) and those from 21 acute myelocytic leukemia patients had low terminal transferase activity (0.2 to 2 units/mg of cellular DNA), the bone marrow and peripheral blood leukocytes from 2 patients with acute myelocytic leukemia, diagnosed on the basis of clinical features and the morphology, cytochemistry, and cytogenetics of the leukemic cells, had terminal transferase activity (39 to 52 units/mg of cellular DNA) equivalent to that found in leukemic lymphoblasts. These results bring under question the specificity of high terminal transferase activity outside of the thymus as a marker for leukemic lymphoblasts and, secondarily, the derivation of acute lymphoblastic leukemia cells in all cases from thymocytes. Perhaps malignant transformation in a pleuripotent stem cell with derepression of the genome for terminal transferase could account for high terminal transferase activity observed in certain leukemic cells.