Cervicovaginal microbiota composition correlates with the acquisition of high-risk human papillomavirus types
Cervicovaginal microbiota composition correlates with the acquisition of high-risk human papillomavirus types
复制标题
宫颈阴道微生物群组成与高危人乳头瘤病毒类型的获得相关
DOI：
10.1002/ijc.31342
复制
发表时间：
2018-08-01
影响因子：
6.4
通讯作者：
Wang, Kai
中科院分区：
文献类型：
--
作者：
Huang, Xiaojie;Li, Chao;Wang, Kai
高危（hr）人乳头瘤病毒（HPV）感染与鳞状上皮内病变（SILs）和宫颈癌的临床状况密切相关。然而，决定hrHPV感染类型的因素仍不清楚。在这里，我们通过对条形码16S rRNA基因的焦磷酸测序，全面研究了280名感染一种hrHPV （HPV 16,52或58）的妇女的宫颈阴道微生物群的细菌组成。在不同SIL组之间和每个组的亚组内观察到不同的微生物群组成。这一结果表明，不是微生物群的多样性或常见的微生物群，而是每种SIL特异性的药物可能对hrHPV类型的获得产生积极影响，而不依赖于丰度。具体来说，宫颈阴道微生物群中的Oribacterium， Lachnobacterium和Thermus的组合物更可能与HPV 16相关，而宫颈阴道微生物群中的Motilibacter的组合物更可能与HPV 52相关，Litorilinea和Paludibaculum的组合物在宫颈阴道微生物群中同时缺乏L. iners更可能与HPV 58相关。此外，关于传染病和癌症相关基因的功能预测显示出显著差异(p
High-risk (hr) human papillomavirus (HPV) infection is closely associated with the clinical conditions of both squamous intraepithelial lesions (SILs) and cervical carcinoma. However, it remains unclear what factors determine the type of hrHPV infection. Here, we have comprehensively investigated the bacterial composition of the cervicovaginal microbiota of 280 women infected with one type of hrHPV (HPV 16, 52 or 58) by the pyrosequencing of barcoded 16S rRNA genes. Differential microbiota composition was observed among various SIL groups and within the subgroups of each group. This result showed that it is not the microbiota diversity or the common microbiota, but rather agents that are specific to each SIL that might have a positive influence on the acquisition of hrHPV types, independent of abundance. Specifically, a composition of Oribacterium, Lachnobacterium and Thermus in the cervicovaginal microbiota is more likely to be associated with HPV 16, while a composition of Motilibacter in the cervicovaginal microbiota is more likely to be associated with HPV 52, and a composition of Litorilinea and Paludibaculum with a concomitant paucity of L. iners in the cervicovaginal microbiota is more likely to be associated with HPV 58. Furthermore, functional predictions regarding infectious diseases and cancer-related genes disclosed significant differences (p