DIVISION AND DIFFERENTIATION OF ISOLATED CNS BLAST CELLS IN MICROCULTURE
DIVISION AND DIFFERENTIATION OF ISOLATED CNS BLAST CELLS IN MICROCULTURE
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DOI：
10.1038/340471a0
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发表时间：
1989-08-10
期刊：
影响因子：
64.8
通讯作者：
TEMPLE, S
中科院分区：
文献类型：
--
作者：
TEMPLE, S
通过研究分离的中枢神经系统（CNS）前体细胞的克隆发育，可以更好地理解神经板的明显相似的细胞转化为成熟脊椎动物CNS 1的众多和不同的细胞类型的机制。在这里，我描述了一个培养系统，其中胚细胞（细胞分裂能力）分离自胚胎13.5-14.5天大鼠前脑可以分裂和分化成各种克隆类型。大多数克隆只含有神经元或神经胶质; 22%含有神经元和非神经元细胞。对于胚细胞的分裂，需要存在活的调节细胞，这表明环境信号影响增殖。异质克隆在同质培养条件下发育，因此母细胞的内在因素可能在决定克隆后代的数量和类型方面很重要。
THE mechanism of transformation of the overtly similar cells of the neural plate into the numerous and diverse cell types of the mature vertebrate central nervous system (CNS)1can better be understood by studying the clonal development of isolated CNS precursor cells. Here I describe a culture system in which blast cells (cells capable of division) isolated from embryonic day 13.5–14.5 rat forebrain can divide and differentiate into a variety of clonal types. Most clones contain only neurons or glia; 22% contain both neurons and non-neuronal cells. For the division of blast cells, live conditioning cells need to be present indicating that environmental signals influence proliferation. Heterogeneous clones develop in homogeneous culture conditions, so factors intrinsic to the blast cells are probably important in determining the number and type of clonal progeny.