INTER-EXPERIMENT VARIATION AND DEPENDENCE ON CULTURE CONDITIONS IN ASSAYING THE CHEMOSENSITIVITY OF HUMAN SMALL-CELL LUNG-CANCER CELL-LINES
INTER-EXPERIMENT VARIATION AND DEPENDENCE ON CULTURE CONDITIONS IN ASSAYING THE CHEMOSENSITIVITY OF HUMAN SMALL-CELL LUNG-CANCER CELL-LINES
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DOI：
10.1016/0277-5379(87)90012-5
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发表时间：
1987-02-01
期刊：
影响因子：
--
通讯作者：
HANSEN, HH
中科院分区：
文献类型：
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作者：
ROED, H;CHRISTENSEN, IJ;HANSEN, HH
采用双层琼脂法测定了5种人小细胞肺癌细胞系对阿霉素的敏感性。这两个底层都没有提供细胞数量与菌落数量之间的比例关系，但它们通过对数函数相关。即使在校正了缺乏比例性后，两种分析系统提供了显着不同的剂量-反应曲线。在体外连续培养25-30周或在液氮中长期保存后检测化学敏感性的稳定性。一个细胞系在连续体外培养后发生了显著的变化，而在液氮中长时间储存后的细胞系只有微小的变化。由此得出结论，在评价新药时，不应考虑在一种细胞系的一次实验中达到一定程度的细胞杀伤（如ID50）所需的浓度，而应采用在不同测定系统中在几种细胞系上获得的剂量-反应曲线。
Sensitivity of five human small cell lung cancer cell lines to doxorubicin was assesed by a double layer agar technique using two different bottom-layers. Neither of the bottom-layers provided proportionality between numbers of cells plated and number of colonies, but they were correlated by a logarithmic function. Even after correction for lack of proportionality the two assay system provided significantly different dose-reponse curves. The stability of the chemosensitivity was tested after 25-30 weeks continuous in vitro culture or prolonged storage in liquid nitrogen. One cell line underwent significant changes after continuous in vitro culture whereas the cell lines tested after prolonged storage in liquid nitrogen showed only minor changes. It is concluded that instead of considering the concentration necessary to achieve a certain degree of cell kill (e.g. ID50) in one experiment on one cell line, dose-response curves obtained on several cell lines in different assay systems should be used in the evaluation of new drugs.