Analysis of WRKY transcription factors and characterization of two Botrytis cinerea-responsive LrWRKY genes from Lilium regale
Analysis of WRKY transcription factors and characterization of two Botrytis cinerea-responsive LrWRKY genes from Lilium regale
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DOI：
10.1016/j.plaphy.2018.04.027
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发表时间：
2018-06-01
影响因子：
6.5
通讯作者：
Jia, Gui-xia
中科院分区：
文献类型：
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作者：
Cui, Qi;Yan, Xiao;Jia, Gui-xia
百合生产中的一个主要限制因素是由椭圆形葡萄孢和B引起的灰霉病。灰叶WRKY转录因子在植物免疫应答中起重要作用。然而，有限的信息是关于百合植物中的WRKY基因家族。在这项研究中，23个LrWRKY基因与完整的WRKY结构域的鉴定从葡萄孢属抗品种丽莲regale。根据WRKY基因的结构特征，将其分为7个亚组（Ⅰ、Ⅱ a-e和Ⅲ组）。序列比对显示，LrWRKY蛋白具有高度保守的WRKYGQK结构域和变体WRKYGKK结构域，并且这些蛋白在整个相同亚组中通常含有相似的基序组合物。功能注释预测它们可能参与与非生物和生物胁迫相关的生物过程。qRT-PCR分析证实了6个LrWRKY基因在L. regale或感病的亚洲杂种'Yale'被B诱导。灰霉病其中LrWRKY 4、LrWRKY 8和LrWRKY 10在L.而LrWRKY 6和LrWRKY 12在L.盛宴此外，还从L.盛宴亚细胞定位分析确定它们靶向于细胞核。拟南芥中LrWRKY 4和LrWRKY 12的组成型表达导致植物对B的抗性更强。比野生型植物。这种抗性与SA和JA响应基因的转录变化相结合。总之，我们的研究提供了有关LrWRKY基因的结构和功能特征的有价值的信息，这不仅将加深我们对百合防御B的分子机制的理解。同时也为通过生物技术进行品种改良提供了潜在的目标。
A major constraint in producing lilies is gray mold caused by Botrytis elliptica and B. cinerea. WRKY transcription factors play important roles in plant immune responses. However, limited information is available about the WRKY gene family in lily plants. In this study, 23 LrWRKY genes with complete WRKY domains were identified from the Botrytis-resistant species Lillian regale. The putative WRKY genes were divided into seven subgroups (Group I, IIa-e, and III) according to their structural features. Sequence alignment revealed that LrWRKY proteins have a highly conserved WRKYGQK domain and a variant, the WRKYGKK domain, and these proteins generally contained similar motif compositions throughout the same subgroup. Functional annotation predicted they might be involved in biological processes related to abiotic and biotic stresses. A qRT-PCR analysis confirmed that expression of six LrWRKY genes in L. regale or the susceptible Asian hybrid 'Yale' was induced by B. cinerea infection. Among these genes, LrWRKY4, LrWRKY8 and LrWRKY10 were expressed at a higher level in L. regale than 'Yale', while the expression of LrWRKY6 and LrWRKY12 was lower in L. regale. Furthermore, LrWRKY4 and LrWRKY12 genes, which also respond to salicylic acid (SA) and methyl jasmonate (MeJA) treatments, were isolated from L. regale. Subcellular localization analysis determined that they were targeted to the nucleus. Constitutive expression of LrWRKY4 and LrWRKY12 in Arabidopsis resulted in plants that were more resistant to B. cinerea than wild-type plants. This resistance was coupled with the transcriptional changes of SA and JA-responsive genes. Overall, our study provides valuable information about the structural and functional characterization of LrWRKY genes that will not only deepen our understanding of the molecular mechanisms underlying the defense of lily against B. cinerea but also offer potential targets for cultivar improvement via biotechnology.