Involvement of CtBP2 in LPS-induced microglial activation
Involvement of CtBP2 in LPS-induced microglial activation
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CtBP2 参与 LPS 诱导的小胶质细胞激活
DOI：
10.1007/s10735-012-9399-x
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发表时间：
2012-06-01
影响因子：
3.2
通讯作者：
Cheng, Chun
中科院分区：
文献类型：
--
作者：
Zhang, Guowei;Yan, Yaohua;Cheng, Chun
CtBP2 （c端结合蛋白2）在发育过程和分化过程中广泛表达，作为转录抑制因子，通过抑制因子或其他协同抑制蛋白募集到目标启动子。在本研究中，我们阐明了CtBP2在脂多糖（LPS）诱导的成年大鼠神经炎症过程中的动态表达变化和定位。CtBP2在炎症脊髓中活跃的胶质细胞（小胶质细胞和星形胶质细胞）中被强烈诱导表达。体外研究表明，CtBP2的上调可能参与了LPS暴露后小胶质细胞的激活。通过siRNA敲除HAPI小胶质细胞CtBP2，表明CtBP2增加了LPS诱导的小胶质细胞活化。综上所述，这些结果表明CtBP2可能在小胶质细胞介导的免疫反应中对宿主防御起重要作用。了解细胞信号通路可能为中枢神经系统炎症免疫反应提供新的策略。
CtBP2 (C-terminal binding protein 2), which is widely expressed during developmental processes and differentiation, acts as a transcriptional repressor following recruitment to target promoters through repressors or other co-repressor proteins. In this study, we elucidated the dynamic expression changes and localization of CtBP2 in lipopolysaccharide (LPS)-induced neuroinflammatory processes in adult rats. CtBP2 expression was strongly induced in active glia cells (microglia and astrocytes) in inflamed spinal cord. In vitro studies indicated that the up-regulation of CtBP2 may be involved in the subsequent microglia activation following LPS exposure. And the knock-down of CtBP2 in microglia cell line HAPI by siRNA showed that CtBP2 increased the activation of microglia induced by LPS. Collectively, these results suggested CtBP2 may be important in host defense in microglia-mediated immune response. Understanding the cell signal pathway may provide a novel strategy against inflammatory and immune reaction in neuroinflammation in central nervous system.