Identification of proteins in human prostate tumor material by two-dimensional gel electrophoresis and mass spectrometry
Identification of proteins in human prostate tumor material by two-dimensional gel electrophoresis and mass spectrometry
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DOI：
10.1007/pl00000858
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发表时间：
2001-02-01
影响因子：
8
通讯作者：
Auer, G
中科院分区：
文献类型：
--
作者：
Alaiya, AA;Oppermann, M;Auer, G
采用双向聚丙烯酰胺凝胶电泳和质谱分析从良性前列腺组织和前列腺癌收集的细胞中的蛋白质模式。通过计算机辅助图像分析（PDQUEST）评估多肽表达。通过凝胶内消化和随后的基质辅助激光解吸/电离质谱法鉴定前列腺肿瘤表达的蛋白质。除了细胞骨架和线粒体蛋白，40 kDa的蛋白被确定为前列腺酸性磷酸酶（PAP）。PAP表达在良性和恶性组织中下降约2倍。热休克蛋白70表达增强，原肌球蛋白1表达减弱。通过二维凝胶电泳和质谱法对前列腺物质的分析表明，特定的蛋白质是作为疾病标志物的感兴趣的蛋白质。
Protein patterns in cells collected from benign prostatic tissues and prostate carcinomas were analyzed using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Polypeptide expression was evaluated by computer-assisted image analysis (PDQUEST). Proteins expressed by prostate tumors were identified via in-gel digestion and subsequent matrix-assisted laser desorption/ionization mass spectrometry. In addition to cytoskeletal and mitochondrial proteins, a 40-kDa protein was identified as prostatic acid phosphatase (PAP). PAP expression decreased approximately twofold between benign and malignant tissue. increased expression of heat shock protein 70 and decreased expression of tropomyosin 1 were also observed in the malignant tissue. The analysis of prostate material by two-dimensional gel electrophoresis and mass spectrometry shows that particular proteins are of interest as markers of disease.