Assignment of Vibrio sp. strain ABE-1 to Colwellia maris sp. nov., a new psychrophilic bacterium.
Assignment of Vibrio sp. strain ABE-1 to Colwellia maris sp. nov., a new psychrophilic bacterium.
复制标题
弧菌的分配。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
H. Okuyama
中科院分区：
文献类型：
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作者：
I. Yumoto;K. Kawasaki;Hideaki Iwata;H. Matsuyama;H. Okuyama
一种嗜冷菌，以前被描述为弧菌属菌株ABE-1 T，已重新分配的表型特征，化学分类学分析和16 S rRNA系统发育分析。该菌为弯曲杆状，能将硝酸盐还原为亚硝酸盐，水解明胶和DNA，但不能水解几丁质。NaCl是生长所必需的。该菌株对抑弧菌化合物O/129敏感。类异戊二烯醌主要为泛醌-8，DNA G + C含量为39.4mol%。全细胞脂肪酸包括具有10-18个C原子的饱和和单不饱和脂肪酸;饱和和单不饱和C16脂肪酸占主导地位。菌株ABE-1 T含有独特的反式不饱和脂肪酸，9-反式十六碳烯酸。虽然菌株ABE-1 T已被鉴定为弧菌属物种，但该菌株不发酵葡萄糖。基于16 S rRNA序列的系统发育分析表明，菌株ABE-1 T与Colwellia种的亲缘关系比弧菌属的亲缘关系近。然而，菌株ABE-1 T不同于其他报道的Colwellia物种的系统发育位置，一些表型特征，化学分类学分析和DNA-DNA杂交的相关性。因此，建议将其命名为Colwellia maris。模式菌株为ABE-1 T（= JCM 10085 T）。
A psychrophilic bacterium, previously described as Vibrio sp. strain ABE-1T, has been reassigned by phenotypic characterization, chemotaxonomic analysis and 16S rRNA phylogenetic analysis. The organism was curved rods and it could reduce nitrate to nitrite and hydrolyse gelatin and DNA, but not chitin. NaCl was required for growth. This strain was susceptible to the vibriostatic compound O/129. The major isoprenoid quinone was ubiquinone-8 and the DNA G + C content was 39.4 mol%. The whole-cell fatty acids comprised saturated and monounsaturated fatty acids with 10-18 C atoms; saturated and monounsaturated C16 fatty acids were predominant. Strain ABE-1T contained the unique trans-unsaturated fatty acid, 9-trans-hexadecenoic acid. Although strain ABE-1T has been identified as a Vibrio species, the strain did not ferment glucose. Phylogenetic analysis based on 16S rRNA sequencing indicated that strain ABE-1T was more closely related to Colwellia species than to Vibrio species. However, strain ABE-1T differed from other reported Colwellia species in terms of phylogenetic position, some phenotypic characteristics, chemotaxonomic analysis and relatedness by DNA-DNA hybridization. Accordingly, the name Colwellia maris is proposed. The type strain is ABE-1T (= JCM 10085T).