Effect of oral bacteria on peripheral blood leukocyte interleukin-6 and soluble interleukin-6 receptor production.
Effect of oral bacteria on peripheral blood leukocyte interleukin-6 and soluble interleukin-6 receptor production.
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口腔细菌对外周血白细胞白细胞介素 6 和可溶性白细胞介素 6 受体产生的影响。
DOI：
10.1111/j.1399-302x.1996.tb00190.x
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发表时间：
1996
影响因子：
--
通讯作者：
Avanessian,AB
中科院分区：
文献类型：
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作者：
Lindemann,RA;KinderHaake,SA;Kjeldsen,M;Avanessian,AB
为了确定口腔致病菌对白细胞介素6（IL-6）和可溶性IL-6受体产生的影响，我们在体外测量了人外周血单核细胞释放它们的情况。将未分离的外周血单个核细胞、外周血淋巴细胞（去除单核细胞）、纯T细胞或单核细胞与伴放线放线杆菌、牙龈嗜二氧化碳噬纤维菌、赭色嗜二氧化碳噬纤维菌、核瘤梭杆菌或牙龈卟啉单胞菌培养24 h。通过酶联免疫吸附试验检测上清液中的IL-6和可溶性IL-6受体。只有单核细胞和外周血单核细胞在所有测试细菌的存在下响应显著的IL-6释放。然而，外周血淋巴细胞在被植物血凝素或IL-2随后被细菌激活时能够产生IL-6，尽管显著低于含有单核细胞的培养物。没有测试的细菌增加可溶性IL-6受体释放超过自发可溶性IL-6受体释放。我们得出结论，单核细胞在接触口腔病原体后释放IL-6;然而，来自T细胞和单核细胞的可溶性IL-6受体是组成性产生的，并可能调节IL-6的作用。
To determine the effect of pathogenic oral bacteria on interleukin 6 (IL‐6) and soluble IL‐6 receptor production, we measured their release by human peripheral blood mononuclear cells in vitro. Unseparated peripheral blood mononuclear cells, peripheral blood lymphocytes (monocyte depleted), pure T cells, or monocytes were cultured withActinobacillus actinomycetemcomitans, Capnocytophaga gingivalis, Capnocytophaga ochracea, Fusobacterium nucleatumorPorphyromonas gingivalisfor 24 h. Supernatants were tested for IL‐6 and soluble IL‐6 receptor by enzyme‐linked immunosorbent assay. Only monocytes and peripheral blood mononuclear cells responded with significant IL‐6 release in the presence of all bacteria tested. However, peripheral blood lymphocytes were capable of producing IL‐6 when activated by phytohemagglutinin or IL‐2 followed by bacteria, though substantially less than cultures containing monocytes. No bacteria tested increased soluble IL‐6 receptor release over spontaneous soluble IL‐6 receptor release. We conclude that monocytes release IL‐6 after contact with oral pathogens; however, soluble IL‐6 receptor from T cells and monocytes is constitutively produced and may modulate EL‐6 actions.