An opioid binding protein is specifically down-regulated by chronic morphine treatment in dorsal root and trigeminal ganglia.
An opioid binding protein is specifically down-regulated by chronic morphine treatment in dorsal root and trigeminal ganglia.
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长期吗啡治疗可特异性下调背根和三叉神经节中的阿片样物质结合蛋白。
DOI：
10.1016/0306-4522(95)00036-i
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发表时间：
1995
期刊：
影响因子：
3.3
通讯作者：
Elde,R
中科院分区：
文献类型：
--
作者：
Kalyuzhny,A;Lee,NM;Elde,R
尽管最近克隆了μ、δ和κ阿片样受体，但阿片样结合细胞粘附分子在阿片样受体功能中的作用得到几条证据的支持，包括阿片样结合细胞粘附分子抗体抑制阿片样结合，培养的NG 108 -15细胞的慢性阿片样激动剂处理下调阿片样结合细胞粘附分子，以及通过转染阿片结合细胞粘附分子反义cDNA减少NG 108 -15细胞中阿片结合。在本研究中，我们报告，慢性在体内治疗的小鼠吗啡的结果在下调阿片结合细胞粘附分子免疫反应性的初级传入神经元在背根和三叉神经节，以及它们的轴突。这种作用可被阿片受体拮抗剂纳洛酮阻断。在中枢神经系统的其他区域未观察到阿片样物质结合细胞粘附分子免疫反应性的下调。两者合计，以前的研究表明阿片受体在调节初级传入神经元的递质释放中所起的作用，以及目前发现的阿片结合细胞粘附分子表达的特异性调节慢性暴露于吗啡，从体内角度提供了证据，提出了阿片结合细胞粘附分子在阿片类药物的作用中发挥作用的概念。
Despite the recent cloning of μ, δ and κ opioid receptors, a role in opioid receptor function for an opioid binding cell adhesion molecule is supported by several lines of evidence, including inhibition of opioid binding by opioid binding cell adhesion molecule antibodies, down-regulation of opioid binding cell adhesion molecule by chronic opioid agonist treatment of cultured NG108-15 cells, and reduction of opioid binding in NG108-15 cells by transfection of opioid binding cell adhesion molecule antisense cDNA. In the present study, we report that chronic in vivo treatment of mice with morphine results in down-regulation of opioid binding cell adhesion molecule immunoreactivity in primary afferent neurons in dorsal root and trigeminal ganglia as well as their axons. This effect was blocked by the opioid antagonist naloxone. Down-regulation of opioid binding cell adhesion molecule immunoreactivity was not observed in other areas of the central nervous system. Taken together, the previous studies which demonstrated the role played by opioid receptors in regulating release of transmitters from primary afferent neurons and the present findings of a specific regulation of opioid binding cell adhesion molecule expression by chronic exposure to morphine, provides evidence from an in vivo perspective which advances the notion that opioid binding cell adhesion molecule plays a role in the action of opioids.