Structural analysis of sphingophospholipids derived from Sphingobacterium spiritivorum, the type species of genus Sphingobacterium
Structural analysis of sphingophospholipids derived from Sphingobacterium spiritivorum, the type species of genus Sphingobacterium
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DOI：
10.1016/j.bbalip.2003.10.010
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发表时间：
2003-12-30
影响因子：
4.8
通讯作者：
Kobayashi, K
中科院分区：
文献类型：
--
作者：
Naka, T;Fujiwara, N;Kobayashi, K
鞘氨醇细菌属的独特特征是除了二酰基甘油磷脂外，还含有神经鞘磷脂和神经酰胺。从鞘氨醇杆菌属的模式种--螺旋鞘氨醇33861(T)中分离纯化出5种鞘氨醇磷脂，它们是主要的细胞脂成分。鉴定为神经酰胺磷酰乙醇胺(CerPE-1和CerPE-2)、神经酰胺磷酰肌醇(CerPI-1和CerPI-2)和神经酰胺磷化甘露糖(CerPM-1)。CerPE-1、CerPI-1和CerPM-1的神经酰胺由15-甲基十六烷基鞘氨醇(iso-C17：0)和13-甲基十四酸(iso-C15：0)组成，而CerPE-2和CerPI-2的神经酰胺由异十七烷和2-羟基-13-甲基十四酸(2-羟基异十五烷酸，2-OH-C15：0)组成。这些鞘磷脂还存在于多毛鞘氨醇杆菌ATCC 33613(T)、水鞘氨醇杆菌ATCC 33299(T)、粪鞘氨醇杆菌IFO 15299(T)、地中海鞘氨醇杆菌ATCC 43320(T)和北极鞘氨醇细菌ATCC 51969(T)中。据我们所知，CerPM-1的存在是一种通过真核和原核细胞的新的鞘氨醇磷脂。(C)2003爱思唯尔B.V.保留所有权利。
The unique feature of the genus Sphingobacterium is the presence of sphingophospholipids and ceramides, besides diacylglycerophospholipids. As major cellular lipid components, five kinds of sphingophospholipids were purified from Sphingobacterium spiritivorum ATCC 33861(T), the type species of genus Sphingobacterium. They were identified as ceramide phosphorylethanolamines (CerPE-1 and CerPE-2), ceramide phosphoryl-myo-inositols (CerPI-1 and CerPI-2), and ceramide phosphorylmannose (CerPM-1). The ceramide of CerPE-1, CerPI-1, and CerPM-1 was composed of 15-methylhexadecasphinganine (isoheptadeca sphinganine, iso-C17:0) and 13-methyltetradecanoic acid (isopentadecanoic acid, iso-C15:0), whereas that of CerPE-2 and CerPI-2 was composed of isoheptadeca sphinganine and 2-hydroxy-13-methyltetradecanoic acid (2-hydroxy isopentadecanoic acid, 2-OH iso-C15:0). These sphingophospholipids were also found in cellular lipids of Sphingobacterium multivorum ATCC 33613(T), Sphingobacterium mizutaii ATCC 33299(T), Sphingobacterium faecium IFO 15299(T), Sphingobacterium thalpophilum ATCC 43320(T), and Sphingobacterium antarcticum ATCC 51969(T). To our knowledge, the existence of CerPM-1 is a novel sphingophospholipid through eukaryotic and prokaryotic cells. (C) 2003 Elsevier B.V. All rights reserved.