Long-Term Experimental Nitrogen Deposition Alters the Composition of the Active Fungal Community in the Forest Floor
Long-Term Experimental Nitrogen Deposition Alters the Composition of the Active Fungal Community in the Forest Floor
复制标题
DOI：
10.2136/sssaj2013.05.0179
复制
发表时间：
2013-09-01
影响因子：
2.9
通讯作者：
Edwards, Ivan P.
中科院分区：
文献类型：
--
作者：
Entwistle, Elizabeth M.;Zak, Donald R.;Edwards, Ivan P.
全球大气氮沉降率的增加有可能改变土壤微生物群落的组成和功能。在这里，我们测试了实验性氮沉降改变了 Dikarya 真菌活跃群落组成的假设。这种变化可能是先前在长期现场实验中观察到的分解减少和土壤有机质增加的基础。活跃代谢的森林地面真菌群落的特征是从 28S 真菌 rRNA 构建的 cDNA 克隆文库中提取的，这些 28S 真菌 rRNA 是从密歇根州下半岛的两个北部阔叶林的森林地面中提取的。我们证明，长期实验性氮沉降改变了每个林分森林地面中 Dikarya 真菌活跃群落的组成。由于森林地表真菌是重要的分解者，因此通过增加氮沉降来改变森林地表真菌群落可能会对森林生态系统中碳的循环和储存产生影响。
Global increases in the rate of atmospheric N deposition have the potential to alter the composition and function of soil microbial communities. Here, we tested the hypothesis that experimental N deposition has altered the composition of active communities of Dikarya fungi. Such a change may underlie previously observed reductions in decomposition and increases in soil organic matter in a long-term field experiment. The actively metabolizing forest floor fungal community was characterized from cDNA clone libraries constructed from 28S fungal rRNA extracted from the forest floor of two northern hardwood stands in the lower peninsula of Michigan. We demonstrate that long-term experimental N deposition altered the composition of the active communities of Dikarya fungi in the forest floor in each of these forest stands. Because forest floor fungi are important decomposers, the alteration of forest floor fungal communities by increasing N deposition may have implications for the cycling and storage of C in forest ecosystems.