Acoustic force mapping in a hybrid acoustic-optical micromanipulation device supporting high resolution optical imaging.
Acoustic force mapping in a hybrid acoustic-optical micromanipulation device supporting high resolution optical imaging.
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DOI：
10.1039/c6lc00182c
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发表时间：
2016-04-21
期刊：
影响因子：
6.1
通讯作者：
Ritsch-Marte M
中科院分区：
文献类型：
--
作者：
Thalhammer G;McDougall C;MacDonald MP;Ritsch-Marte M
我们展示了结合声光捕获与透明压电换能器支持高分辨率成像和声力映射。生命科学中的许多应用都需要非接触式操作工具，以实现对各种类型样品的有力但又精细的处理。此外，该系统应支持高分辨率光学成像。在这里，我们提出了一种混合声/光操纵系统，它利用透明的换能器，使其与高NA成像在微流体环境中兼容。分层谐振器内强大的声学捕获功能适用于高度平行的颗粒处理，全息光镊的灵活性和选择性则是其补充，样品始终处于高质量的光学监控之下。该系统的双重声学/光学性质使其能够通过对光学捕获的颗粒进行直接力测量来光学测量精确的声学力图。对于对力测量的精度具有（超）高要求的应用，用于高NA成像的物镜的位置可能对探针室中的声学力图具有显著影响。我们的特点是这种影响的实验和模型模拟的结果得到证实。我们表明，它是可能的设计室，并选择在这样一种方式，以避免由于物镜透镜扰动的工作点。此外，我们发现，测量换能器的电阻抗提供了一个简单的指标的声共振。
We demonstrate combined acoustic-optical trapping with transparent piezoelectric transducers supporting high-resolution imaging and acoustic force mapping. Many applications in the life-sciences demand non-contact manipulation tools for forceful but nevertheless delicate handling of various types of sample. Moreover, the system should support high-resolution optical imaging. Here we present a hybrid acoustic/optical manipulation system which utilizes a transparent transducer, making it compatible with high-NA imaging in a microfluidic environment. The powerful acoustic trapping within a layered resonator, which is suitable for highly parallel particle handling, is complemented by the flexibility and selectivity of holographic optical tweezers, with the specimens being under high quality optical monitoring at all times. The dual acoustic/optical nature of the system lends itself to optically measure the exact acoustic force map, by means of direct force measurements on an optically trapped particle. For applications with (ultra-)high demand on the precision of the force measurements, the position of the objective used for the high-NA imaging may have significant influence on the acoustic force map in the probe chamber. We have characterized this influence experimentally and the findings were confirmed by model simulations. We show that it is possible to design the chamber and to choose the operating point in such a way as to avoid perturbations due to the objective lens. Moreover, we found that measuring the electrical impedance of the transducer provides an easy indicator for the acoustic resonances.
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影响因子：
2.4
作者：
Barnkob, Rune;Augustsson, Per;Bruus, Henrik
通讯作者：
Bruus, Henrik
影响因子：
3.3
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Candelli, Andrea;Wuite, Gijs J. L.;Peterman, Erwin J. G.
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Peterman, Erwin J. G.
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Thalhammer, Gregor;Obmascher, Lisa;Ritsch-Marte, Monika
通讯作者：
Ritsch-Marte, Monika
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3.8
作者：
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通讯作者：
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影响因子：
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作者：
Lakaemper, Stefan;Lamprecht, Andreas;Dual, Jurg
通讯作者：
Dual, Jurg