Molecular epidemiology of acquired-metallo-β-lactamase-producing bacteria in Poland
Molecular epidemiology of acquired-metallo-β-lactamase-producing bacteria in Poland
复制标题
DOI：
10.1128/aac.50.3.880-886.2006
复制
发表时间：
2006-03-01
影响因子：
4.9
通讯作者：
Gniadkowski, M
中科院分区：
文献类型：
--
作者：
Fiett, J;Baraniak, A;Gniadkowski, M
我们分析了2000年至2004年在波兰12个城市的17家医院发现的40株产生金属β -内酰胺酶（MBL）的铜绿假单胞菌（n = 38）、恶臭假单胞菌（n = 1）和不动杆菌3基因种（n = 1）。脉冲场凝胶电泳分型将铜绿假单胞菌分为8种类型，其中2种类型进一步分化为亚型。每种类型都是特定于一个给定的中心或同一或邻近地理区域的几家医院。几乎所有的生物体都产生β -内酰胺酶VIM-2；唯一的例外是两个中心的铜绿假单胞菌分离株表达VIM-4。bla（VIM）基因仅存在于1类整合子中，这些整合子位于染色体或质粒DNA中。整合子可变区域的pcr -限制性片段长度多态性研究以及DNA测序显示，存在8个不同的，大多数是新的基因盒阵列，其中6个包含bla(VIM-2)， 2个包含bla（VIM-4）。整合子变体的出现与产生mbl的生物的地理分布密切相关，这表明它们在该国特定地区的出现可能是由于许多独立事件。以下MBL生产者的区域传播可归因于各种现象，包括它们的克隆传播，抗性决定因素的水平传播，或两者兼而有之。本研究收集的所有数据表明，即使在发现的早期阶段，波兰MBL生产者的流行病学情况已经非常复杂和动态。
We have analyzed 40 metallo-beta-lactamase (MBL)-producing isolates of Pseudomonas aeruginosa (n = 38), Pseudomonas putida (n = 1), and Acinetobacter genospecies 3 (n = 1) from 17 hospitals in 12 cities in Poland that were identified in 2000 to 2004. Pulsed field gel electrophoresis typing classified the P. aeruginosa isolates into eight types, with two types differentiated further into subtypes. Each of the types was specific either to a given center or to several hospitals of the same or neighboring geographic area. Almost all of the organisms produced beta-lactamase VIM-2; the only exceptions were several P. aeruginosa isolates from two centers which expressed VIM-4. The bla(VIM) genes resided exclusively within class 1 integrons, and these were located in either chromosomal or plasmid DNA. PCR-restriction fragment length polymorphism study of the variable regions of the integrons, followed by DNA sequencing, revealed the presence of eight different, mostly novel gene cassette arrays, six of which contained bla(VIM-2) and two of which contained bla(VIM-4). The occurrence of the integron variants correlated well with the geographic distribution of the MBL-producing organisms, and this suggested that their emergence in particular parts of the country had been likely due to a number of independent events. The following regional dissemination of MBL producers could be attributed to various phenomena, including their clonal spread, horizontal transmission of resistance determinants, or both. All of the data collected in this study revealed that even at this early stage of detection, the epidemiological situation concerning MBL producers in Poland has already been complex and very dynamic.