Improvement of terrestrial groundwater sampling method affects microbial community analysis
Improvement of terrestrial groundwater sampling method affects microbial community analysis
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DOI：
10.1080/01490451.2018.1534900
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发表时间：
2017-10
影响因子：
2.3
通讯作者：
Akio Ueno;Satoshi Tamazawa;S. Tamamura;Noritaka Aramaki;A. K. M. Alam Badrul;T. Murakami;S. Yamaguchi;J. Yamagishi;H. Tamaki;D. Mayumi;T. Naganuma;K. Kaneko
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文献类型：
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作者：
Akio Ueno;Satoshi Tamazawa;S. Tamamura;Noritaka Aramaki;A. K. M. Alam Badrul;T. Murakami;S. Yamaguchi;J. Yamagishi;H. Tamaki;D. Mayumi;T. Naganuma;K. Kaneko
摘要地下水采样是地下微生物生态学研究的关键步骤。在这里，我们比较了两种不同的采样方法：常用的一次性水瓢（未改进的采样器）和改进的采样器，后者的目的是尽量减少暴露于有氧大气。利用16S rRNA和甲基辅酶M还原酶（mcrA）基因对褐煤煤层地下水中的微生物群落进行分析，结果表明，改良采样器采集的样品中古菌群落以氢营养型产甲烷菌甲烷杆菌为主。这些结果表明，改进后的采样器比未改进的采样器更有利于获得产甲烷古菌群落，采样方法影响了所调查地下褐煤煤层中微生物群落的分析。
Abstract Groundwater sampling is a critical step in subsurface microbial ecology. Here, we compared two different sampling methods: commonly used disposable bailers (unimproved sampler) and an improved sampler, the latter of which was devised to minimize exposure to the aerobic atmosphere. Microbial community analysis using the 16S rRNA and methyl coenzyme-M reductase (mcrA) genes in the lignite seam groundwater revealed that the archaeal communities in samples obtained by the improved sampler were dominated by hydrogenotrophic methanogen Methanobacterium. These results suggested that the improved sampler would be more favorable for obtaining methanogenic archaeal community than the unimproved one, and that the sampling method affected the microbial community analysis in the investigated subterranean lignite seams.